畜牧兽医学报2017，Vol.48Issue(8)：1401-1415,15.DOI:10.11843/j.issn.0366-6964.2017.08.004
马鹿鹿茸不同部位差异蛋白质组学分析
Comparative Proteomics Analysis on Different Parts of Cervus elaphus songaricus Velvet Antler
摘要
Abstract
This study aimed to provide theoretical basis for clarifying velvet antler biological characteristics by comparative proteomics.In this study,different parts of Cervus elaphus songaricus velvet antler (on 65 and 75 days old) were studied by two-dimensional gel electrophoresis,MAL-DI-TOF-MS mass spectrometry identification and bioinformatics analysis.65 differentially expressed proteins were successfully identified,and these proteins were involved in the cellular process,developmental process,localization,metabolic process,response to stimulus,and the KEGG pathway of platelet activation,focal adhesion,metabolic pathways,PI3K-Akt signaling pathway.These proteins were associated with bone development (4 proteins),nerve development (3 proteins),vascular development (2 proteins),anti-oxidation and endoplasmic reticulum stress (4 proteins),immune (7 proteins),apoptosis (5 proteins).The results indicated that TTR,RBP4 and CRABP1 played important roles in regulating the growth and development of velvet antler in the process of antler's transition from 65 d to 75 d velvet antler.PRDX2,TXNDC5,ERP29,Pdia6 played important roles in endoplasmic reticulum stress and anti-oxidation.Endoplasmic reticulum stress,endoplasmic reticulum-related death pathway and mitochondrial apoptotic pathways could explain the rapid differentiation of velvet antler cells while a large amount of apoptosis,which could meet the rapid growth and development needs of velvet antler.In addition,GMFβ and CATHL1 may play important roles in nerve development and anti-inflammatory.关键词
天山马鹿/鹿茸/蛋白质组学/双向凝胶电泳Key words
Cervus elaphus songaricus/velvet antler/proteomics/2-DE分类
农业科技引用本文复制引用
王磊,张然然,刘华淼,刘汇涛,周永娜,董世武,邢秀梅..马鹿鹿茸不同部位差异蛋白质组学分析[J].畜牧兽医学报,2017,48(8):1401-1415,15.基金项目
中国农业科学院科技创新工程“特种动物遗传资源创新团队”(CAAS-ASTIP-201X-ISAPS) （CAAS-ASTIP-201X-ISAPS）
特种动物种质资源平台 （）
