畜牧兽医学报2017，Vol.48Issue(8)：1416-1423,8.DOI:10.11843/j.issn.0366-6964.2017.08.005
miR-194-5p靶向调控BCKDHA基因的表达
miR-194-5p Targets BCKDHA Gene to Regulate Its Expression
摘要
Abstract
This study aimed to predict and validate the crucial miRNA regulating BCKDHA gene expression,where BCKDHA was α subunit of BCKDH (Branched-chain α-ketoacid dehydrogenase complex),which was a key rate-limiting enzyme in the catabolism of branched-chain amino acids.Three online softwares were used to predict miRNAs targeting BCKDHA.QPCR was used to detect expressions of the crucial miRNA and BCKDHA mRNA in 3T3-L1 cells during its differentiation and after over-expressing the crucial miRNA,respectively.The results showed that miR-194-5p was a key miRNA regulating BCKDHA expression with fully complementation between its seed sequence and the 190-196th bases of BCKDHA 3'UTR.The expression of miR-194-5p decreased while that of BCKDHA mRNA increased with differentiation.The target relationship was also validated by dual luciferase reporter results which showed that miR-194-5p negatively regulated the expression of BCKDHA gene.The expression of BCKDHA mRNA was significantly reduced(P＜0.05) after over-expressing miR-194-5p.It is concluded that miR-194-5p inhibits the expression of BCKDHA by targeting its 3'UTR.关键词
miR-194-5p/BCKDHA基因/miRNA预测/3T3-L1/靶基因验证Key words
miR-194-5p/BCKDHA gene/miRNA prediction/3T3-L1/target gene validation分类
农业科技引用本文复制引用
张莹姣,乔利英,景炅婕,潘洋洋,马元,郭云利,魏琼,刘文忠..miR-194-5p靶向调控BCKDHA基因的表达[J].畜牧兽医学报,2017,48(8):1416-1423,8.基金项目
国家自然科学基金项目(31372292) （31372292）
