畜牧兽医学报2017，Vol.48Issue(8)：1424-1435,12.DOI:10.11843/j.issn.0366-6964.2017.08.006
长链非编码RNA HOTAIR的分析及在黑色素瘤致病过程中互作分子的预测
Analysis of Long Non-coding RNA HOTAIR and Prediction of Its Interaction Molecules in Melanoma Pathogenesis
摘要
Abstract
This study aimed to predict the transcription factor,protein and miRNA interacting with HOTAIR by bioinformatics,provide research direction for understanding the molecular mechanism of melanoma pathogenesis,and provide a target for designing new drugs and a theoretical basis for treating the melanoma.In this study,we downloaded HOTAIR sequences of 8 species from UCSC database,established phylogenetic tree by MEGA4.0.We used lncRNAtor and other databases to analyze the upstream regulatory factors,miRNA interacting with HOTAIR and related proteins.The expression patterns of HOTAIR in various tissues and the diseases associated with HOTAIR were analyzed via NONCODE and lncDisease databases.Some of the target miRNAs related to melanoma were predicted by TargetScan software.The molecular regulatory network of miRNA-HOTAIR-melanoma-associated genes was drew using Cytoscape3.2.0 software.The results showed that HOTAIR was located on human 12q13.13 chromosome between HOXC11 and HOXC12 genes at 54 356 092-54 368 740 nt.The HOTAIR was conservative among different species.HOTAIR was regulated by 27 regulatory factors in 18 different cells.HOTAIR was expressed in various tissues,and the expression in testis was higher than that in other tissues,but the expression in lung and ovary was lower than that in other tissues.GO analysis showed that the target genes of HOTAIR involved in cell apoptosis,DNA replication,transcription and other biological processes,which showed that HOTAIR played important roles in the process of pathology and physiology of cancer and some human diseases.The interaction analysis showed that there were interaction sites in miR-217,miR-203 and miR-194 with HOTAIR.In melanoma,HOTAIR may compete with MITF and NKX3-1 to miR-217,miR-203 and miR-194.GO function analysis and KEGG pathway analysis show that the expression level of HOTAIR was closely related to tumor metastasis,recurrence and prognosis,combined with the regulation of transcription factors to HOTAIR,which provide a theoretical basis for treatment of tumors.关键词
长链非编码RNA/HOTAIR/生物信息学/黑色素瘤Key words
long non-coding RNA/HOTAIR/bioinformatics/melanoma分类
农业科技引用本文复制引用
李园,刘文艳,蔡永强,张利环,李宏,朱芷葳..长链非编码RNA HOTAIR的分析及在黑色素瘤致病过程中互作分子的预测[J].畜牧兽医学报,2017,48(8):1424-1435,12.基金项目
国家自然科学基金(31402156) （31402156）
山西省基础研究项目(2014021028-2) （2014021028-2）
山西省科技重点研发项目(2015-TN-10) （2015-TN-10）
山西农业大学青年拔尖创新人才支持计划(TYIT201403) （TYIT201403）
