畜牧兽医学报2017，Vol.48Issue(8)：1468-1480,13.DOI:10.11843/j.issn.0366-6964.2017.08.011
体外消化法优化生长猪玉米-豆粕-DDGS饲粮和小麦-豆粕饲粮非淀粉多糖酶谱的研究
Optimization of Non-starch Polysaccharide Enzymes of Corn-Soybean-DDGS and Wheat-Soybean Diets for Growing Pig Using in vitro Method
摘要
Abstract
The study was conducted to optimize non-starch polysaccharide(NSP) enzymes of cornsoybean-DDGS diet and wheat-soybean diet for growing pigs using in vitro method.The NSP enzymes (cellulase,xylanase,β-mannanase,α-galactosidase,β-glucanase and pectinase) were added into the corn-soybean-DDGS diet and the wheat-soybean diet.In a one-way completely randomized experimental design,in vitro dry matter digestibility (IVDMD) of the two diets was determined using the gastric fluid and small intestinal fluid based on physiological data of growing pigs and the dose-response of the NSP enzymes on the IVDMD of diets were analyzed,respectively.Then the regression relationship of 6 NSP enzymes and the IVDMD of diets were established and the optimal NSP enzyme components was screened using a quadratic regress-universal rotary design.The results showed that:1) Quadratic effects of NSP enzymes on the IVDMD of corn-soybean-DDGS and wheat-soybean diets for growing pig were observed in the present study.2) The optimal NSP enzyme components were 427.1 U · kg-1 cellulase,7 057.5 U · kg-1 xylanase,3 587.8 U · kg-1β-mannanase,202.1 U · kg-1α-galactosidase,1 543.3 U · kg-1 β-glucanase and 72.7 U · kg-1 pectinase for corn-soybean-DDGS diet,and 1 117.9 U · kg-1 cellulase,35 087.7 U· kg-1 xylanase,1 917.1 U · kg-1β-mannanase,305.0 U · kg-1α-galactosidase,806.7 U · kg 1β-glucanase and 133.7 U · kg-1 pectinase for wheat-soybean diet.3) The IVDMD of corn-soybean-DDGS diet and wheat-soybean diet with the optimal NSP enzymes were increased by 3.89％ and 3.48％,respectively.The optimal NSP enzyme components effectively improve the IVDMD of corn-soybean-DDGS diet and wheat-soybean diet.The in vitro digestion method based on physiological parameter of growing pigs can be used as a convenient and rapid method for screening NSP enzyme components of a swine diet.关键词
体外法/非淀粉多糖酶/优化/生长猪Key words
in vitro method/non-starch polysaccharides enzyme/optimization/growing pig分类
农业科技引用本文复制引用
张立兰,高理想,陈亮,钟儒清,张宏福..体外消化法优化生长猪玉米-豆粕-DDGS饲粮和小麦-豆粕饲粮非淀粉多糖酶谱的研究[J].畜牧兽医学报,2017,48(8):1468-1480,13.基金项目
中央级公益性科研院所基本科研业务费专项(2017ywf-zd-18) （2017ywf-zd-18）
中国农业科学院科技创新工程(ASTIP-IAS07) （ASTIP-IAS07）
