畜牧兽医学报2017，Vol.48Issue(8)：1505-1510,6.DOI:10.11843/j.issn.0366-6964.2017.08.015
一株牛源污蝇杆菌的分离鉴定、耐药性及耐药基因检测
Isolation, Identification, Drug Resistance and Detection of Drug Resistance Genes of Bovine Wohlfahrtiimonas chitiniclastica
摘要
Abstract
Wohlfahrtiimonas chitiniclastica is a kind of emerging,rare zoonotic pathogen.In October of 2016,a head of fattening cattle from a beef cattle farm of Jilin Province showed appear purulent lesions in the eyelid,and a variety of antimicrobial treatments are not effective.Eyelid secretion of diseased cattle were collected to isolate and culture bacteria.According to culture,staining,biochemical test and 16S rRNA sequence analysis,the isolated strain was identified as Wohlfahrtiimonas chitiniclastica,and it was pathogenic to mice.The resistance of the isolate was determined by K-B disk diffusion method using 20 antibiotics.The results showed that the isolate was resistant to penicillin,cefradine and erythromycin,and it was sensitive to the other 17 drugs.Then the β-lactam resistance gene (TEM,ADC,OXA-23,OXA-51),macrolide resistance genes (ermA,ermX) and tetracycline resistance genes (tetK,tetW) were detected,respectively,and the results were negative.关键词
污蝇杆菌/分离鉴定/耐药性/耐药基因Key words
Wohlfahrtiimonas chitiniclastica/isolation and identification/drug resistance/resistance gene分类
农业科技引用本文复制引用
李雪嵩,张喜庆,董文龙,王羽,张红伟,田佳琪,马红霞,高云航..一株牛源污蝇杆菌的分离鉴定、耐药性及耐药基因检测[J].畜牧兽医学报,2017,48(8):1505-1510,6.基金项目
吉林省现代农业产业技术体系建设专项(2015-24) （2015-24）
吉林省动物疾病防治研究创新团队(20111820) （20111820）
国家科技支撑计划项目(2014BAK19B005) （2014BAK19B005）
吉林省科技成果转化项目(20140309012NY) （20140309012NY）
