畜牧兽医学报2017，Vol.48Issue(8)：1519-1528,10.DOI:10.11843/j.issn.0366-6964.2017.08.017
细粒棘球绦虫原头蚴蛋白表达谱分析
Protein Profile Analyses of Protoscoleces in Echinococcus granulosus
摘要
Abstract
The aim of this study was to explore the protein expression characteristics of protoscoleces,to reveal the main functional proteins and signaling pathways during the development of protoscoleces in Echinococcus granulusos,to lay the foundation for the mechanisms of development regulation of protoscoleces and screening target genes for vaccines and drugs.The LC-MS/ MS technology was used to analyze the proteins expressed in the protoscoleces,then the proteins were analyzed based on GO,KOG and KEGG.Finally,the qRT-PCR and in situ hybridization were used to analyze the different expression and localization of the β-catenin which is the key gene of the Wnt signaling pathway.The results showed that the present study identified 7 172 peptides,1 197 proteins,including multiple potential antigenic proteins,such as tetraspanin superfamily,6-phosphogluconate dehydrogenase,enolase and epidermal antigenic proteins.Signal pathway analysis showed that 632 of these proteins involved in 276 pathways,such as Wnt,Notch,Hedgehog,NF-κB,cAMP and bile acid signaling pathways closely related to development of E.granulosus.The in situ hybridization analysis showed that the β-catenin was mainly distributed in the hooks and dispersed cells of the protoscoleces.Meanwhile,the relative transcription of β-catenin was gradually decreased during 0 to 10 days in vitro culture.In summary,this study analyzed the protein expression elements of protoscoleces,revealed the main regulatory pathways of protoscolex development,and provided a theoretical basis for the growth mechanisms of protoscoleces and effective prevention of hydatid disease.关键词
细粒棘球绦虫/原头蚴/蛋白/表达谱/信号通路Key words
Echinococcus granulosus/protoscolex/protein/profile/signaling pathway分类
农业科技引用本文复制引用
王正荣,薄新文,张艳艳,马勋,王智欣,卢平萍..细粒棘球绦虫原头蚴蛋白表达谱分析[J].畜牧兽医学报,2017,48(8):1519-1528,10.基金项目
国家重点基础研究发展计划(973计划)项目(2015CB150300) （973计划）
国家自然科学基金(31360608) （31360608）
新疆生产建设兵团科技攻关项目(2014BA002) （2014BA002）
新疆生产建设兵团国际科技合作项目(2013BC001) （2013BC001）
新疆生产建设兵团绵羊繁育生物技术重点实验室开放课题(2013KLS06) （2013KLS06）
