畜牧兽医学报2017，Vol.48Issue(8)：1529-1534,6.DOI:10.11843/j.issn.0366-6964.2017.08.018
不同异嗜性粒细胞与淋巴细胞比值雏鸡对肠炎沙门菌抗性差异的分析
The Analysis of the Difference of Salmonella Resistance between High and Low Heterophil/Lymphocyte Ratio in Chickens
摘要
Abstract
This study was conducted to reveal the difference of immune response upon Salmonella enteritidis (SE) infection between chickens with different Heterophil and Lymphocyte ratio,and trying to prove that chicken flocks of low H/L values has better resistance to Salmonella.Chicken with high and low H/L ratio were selected as parents,and the offspring were divided as High H/L Group and Low H/L Group (HG and LG).The ratio of male and female is 1:3.The chicks were challenged with SE at 7 days old.Liver,cecum and blood serum were collected at 1,2,4,7 and 13 dpi (days post infection).Our results showed that the mortality rate of LG (16.7％) was significantly lower than HG (22.9％).The CFU of SE in liver were significantly different between HG and LG (P＜0.05) at 1,2,4 and 7 dpi.IL-6 in serum were remarkably higher in Low H/L group than in high group (P＜0.05) at 1,2 and 4 dpi.Low H/L chickens had more TNF-α and LTB-4 in serum than high H/L chickens (P＜0.05) at 1,2 dpi.Chicken with low H/ L ratio might have stronger immune response against bacterial infection.关键词
雏鸡/肠炎沙门菌/死亡率/组织载菌量/血清介质Key words
chicken/Salmonella enteritidis/mortality rate/bacteria burden of tissue/serum inflammation media分类
农业科技引用本文复制引用
营凡,赵桂苹,李鹏,邢思远,李庆贺,郑麦青,刘冉冉,崔焕先,文杰,马友记..不同异嗜性粒细胞与淋巴细胞比值雏鸡对肠炎沙门菌抗性差异的分析[J].畜牧兽医学报,2017,48(8):1529-1534,6.基金项目
中国农业科学院科技创新工程(ASTIP-IAS04) （ASTIP-IAS04）
国家科技支撑项目(2015BAD03B03) （2015BAD03B03）
国家自然科学基金(31572393) （31572393）
