医药导报2017，Vol.36Issue(8)：865-869,5.DOI:10.3870/j.issn.1004-0781.2017.08.006
姜黄素对非小细胞肺癌A549细胞的影响
Effect of Curcumin on Non-Small Cell Lung Cancer A549 Cells
摘要
Abstract
Objective To study the inhibitory effect of curcumin on the proliferation,migration and invasion of non-small cell lung cancer cell A549,and to discuss further if it is closely related to the expression of c-Jun N-terminal kinase (JNK) and relative protein p38.Methods A549 cells were cultured by conventional method,and then treated with different concentration of curcumin (10,20,40,80 μmol · L-1).The proliferation,migration and invasion of A549 cells were measured by real-time cellular analysis (RTCA).The expression levels of JNK,p-JNK,p38 and P-p38 were detected by real-time PCR and Western blotting.Results Curcumin showed an antiproliferation effect against A549 cells with IC50 =40 μmol · L-1,and curcumin exhibited obviously inhibitory effect on the migration and invasion of A549 cells.Additionally,compared with control group,curcumin suppressed the expression of JNK and p38 at the gene level,and significantly inhibited the expression of JNK,P-JNK,p38 and p38 (P＜0.05) at the protein level.Conclusion These results demonstrated that curcumin can inhibit the proliferation,migration and invasion of A549 cells via reducing the level of JNK,p38 phosphorylation,and blocking JNK signal transduction pathway.关键词
姜黄素/癌,肺,非小细胞/c-Jun氨基末端激酶/相关蛋白p38Key words
Curcumin/Cancer, lung, non-small cell/c-Jun N-terminal kinase/Relative protein p38分类
医药卫生引用本文复制引用
刘俊香,刘洁婷,唐春银,李玲玉,柏合,袁晓环,长伟,孙丽岩,武艳..姜黄素对非小细胞肺癌A549细胞的影响[J].医药导报,2017,36(8):865-869,5.基金项目
黑龙江省教育厅科学技术研究项目(12541842) （12541842）
黑龙江省卫生厅科研课题(2013199) （2013199）
牡丹江医学院2014年度研究生创新科研项目(2014YJSCX-19) （2014YJSCX-19）
