中国当代儿科杂志2017，Vol.19Issue(9)：1003-1007,5.DOI:10.7499/j.issn.1008-8830.2017.09.014
少突胶质前体细胞移植治疗早产儿脑白质损伤大鼠模型
Long-term effect of oligodendrocyte precursor cell transplantation on a rat model of white matter injury in the preterm infant
摘要
Abstract
Objective To investigate the long-term effect of oligodendrocyte precursor cell (OPC) transplantation on a rat model of white matter injury (WMI) in the preterm infant. Methods A total of 80 Sprague-Dawley rats aged 3 days were randomly divided into sham-operation group, model control group, 5-day ventricular/white matter transplantation group, 9-day ventricular/white matter transplantation group, 14-day ventricular/white matter transplantation group (n=10 each). All groups except the sham-operation group were treated with right common carotid artery ligation and hypoxia for 80 minutes to establish a rat model of WMI in the preterm infant. OPCs were prepared from the human fetal brain tissue (10-12 gestational weeks). At 5, 9, and 14 days after modeling, 3×105 OPCs were injected into the right lateral ventricle or white matter in each transplantation group, and myelin sheath and neurological function were evaluated under an electron microscope at ages of 60 and 90 days. Results Electron microscopy showed that at an age of 60 days, each transplantation group had a slight improvement in myelin sheath injury compared with the model control group; at an age of 90 days, each transplantation group had significantly thickened myelin sheath and reduced structural damage compared with the model control group, and the 14-day transplantation groups had the most significant changes. There were no significant differences in the degree of myelin sheath injury between the ventricular and white matter transplantation groups at different time points. At an age of 60 or 90 days, the transplantation groups had a significantly higher modified neurological severity score (mNSS) than the sham-operation group and a significantly lower mNSS than the model control group (P<0.05). Conclusions OPC transplantation may have a long-term effect in the treatment of WMI in the preterm infant, and delayed transplantation may enhance its therapeutic effect.关键词
早产儿脑白质损伤/少突胶质前体细胞/细胞移植/髓鞘/新生大鼠Key words
White matter injury in the preterm infant/Oligodendrocyte precursor cell/Cell transplantation/Myelin sheath/Neonatal rats引用本文复制引用
吴成君,汪兆艳,杨印祥,栾佐..少突胶质前体细胞移植治疗早产儿脑白质损伤大鼠模型[J].中国当代儿科杂志,2017,19(9):1003-1007,5.基金项目
国家国际科技合作专项资助(2012DFA30880). （2012DFA30880）
