中国生物防治学报2017，Vol.33Issue(4)：446-453,8.DOI:10.16409/j.cnki.2095-039x.2017.04.003
鳢肠乙醇粗提物与Cry1Ca的协同增效及对甜菜夜蛾SeABCC1-SeABCC3mRNA表达量的影响
Synergism between Crude Ethanol Extract of Eclipta prostrata with Cry1Ca and Its Influence on the Expression of ATP-binding Cassette Transporters C mRNA in Spodoptera exigua
摘要
Abstract
The objective of this study was to determine the synergistic effect of crude ethanol extract of Eclipta prostrata (CEEEP) on toxicity of CrylCa against Spodoptera exigua and the changes of expression of SeABCC1,SeABCC2 and SeABCC3.The results showed that CEEEP had an obvious poisoning effect on the 2nd instar larvae ofS.exigua.When fed 5.26 mg/cm2 of CEEEP for 7 days,the mortality rate was 58.4％.When S.exigua ingested the admixture of LD30 of CEEEP and CrylCa,larval mortalities were 59.72％,73.61％ and 83.33％ after 3 d,5 d and 7 d.However,with LD30 of CrylCa alone,the mortalities were 5.6％,13.9％,31.9％,respectively,and with CEEEP alone,the mortalities were 21.8％,28.7％,30.1％,respectively.The relative expression levels of SeABCC1,SeABCC2 and SeABCC3 were analyzed by qPCR,CEEEP can induce high expression of SeABCC1,SeABCC2 and SeABCC3,causing the larvae ofS.exigua more susceptibility to CrylCa.In summary,CEEEP as botanical pesticide can be mixed with Bt toxin,to improve the control effect against S.exigua.关键词
鳢肠乙醇粗提物/Cry1Ca/甜菜夜蛾/毒力/ABC转运蛋白/增效Key words
crude ethanol extract of Eclipta prostrata/Cry1Ca/Spodoptera exigua/toxicity/ATP-binding cassette transporters C/synergism分类
农业科技引用本文复制引用
王莹莹,任相亮,姜伟丽,胡红岩,马亚杰,李倩,马艳..鳢肠乙醇粗提物与Cry1Ca的协同增效及对甜菜夜蛾SeABCC1-SeABCC3mRNA表达量的影响[J].中国生物防治学报,2017,33(4):446-453,8.基金项目
国家自然科学基金(31601648) （31601648）
转基因生物新品种培育重大专项(2016ZX08011-002) （2016ZX08011-002）
