中国生物防治学报2017，Vol.33Issue(4)：463-471,9.DOI:10.16409/j.cnki.2095-039x.2017.04.005
棉花转甲基酶基因GhBSMT1的克隆、原核表达及表达谱分析
Cloning and Prokaryotic Expression of the Methyltransferase Gene GhBSMT1 from Gossypium hirsutum and Its Expression Profile Analysis
摘要
Abstract
In this study,a novel benzoic acid/salicylic acid carboxyl methyltransferase gene was cloned from Gossypium hirsutum CCRI 12 by using RT-PCR and RACE-PCR,which contained 1280 bp and named as GhBSMT1.The transferase was predicted to have a molecular weight of 40.8 kD and isoelectric point of 6.12,which was unstable hydrophilic protein.A recombinant soluble protein with a size of about 41 kD was also obtained by prokaryotic expression.The real-time quantitative PCR analysis indicated that GhBSMT1 was constitutively expressed in leaves,stems and roots of cotton plants.The transcript accumulation of GhBSMT1 in leaves declined significantly when the leaves were treated with methyl salicylate (MeSA),whereas methyl jasmonate (MeJA) had no obvious influence on the expression of GhBSMT1.Moreover,the expressions of GhBSMT1 were reduced significantly after 6 h or 48 h feeding by Helicoverpa armigera larvae.In general,the GhBSMT1 is expressed in all tissues of cotton plants and regulated by MeSA and H.armigera infestation.关键词
陆地棉/苯甲酸/水杨酸羧基甲基转移酶/序列特征/原核表达/组织表达谱Key words
Gossypium hirsutum/benzoic acid/salicylic acid carboxyl methyltransferases/sequence characteristics/prokaryotic expression/tissue expression profile分类
农业科技引用本文复制引用
井维霞,黄欣蒸,刘丹凤,张强,寇俊凤,曲爱军,张永军,郭予元..棉花转甲基酶基因GhBSMT1的克隆、原核表达及表达谱分析[J].中国生物防治学报,2017,33(4):463-471,9.基金项目
国家自然科学基金(31471778,31672038,31621064) （31471778,31672038,31621064）
