中国生物防治学报2017，Vol.33Issue(4)：472-480,9.DOI:10.16409/j.cnki.2095-039x.2017.04.006
两近缘种茶尺蠖的抗菌肽基因在抵抗EoNPV感染过程中的表达差异
The Expression of the Antibacterial Peptide Genes from Two Sibling Species of Tea Geometrid was Different in Resistance to EoNPV Infection
摘要
Abstract
Ectropis obliqua and Ectropis grisescens are two sibling species,which are severe defoliator pests in tea plantations.The mortality rate of E.obliqua and E.grisescens were 100％ and 35.5％ after infection with the E.obliqua nucleopolyhedrovirus (EoNPV) for 10 days,respectiv.ely.Three antimicrobial peptides genes,including gloverin,attacin and moricin,were cloned from the third-instar larvae of the two infected sibling species by an overlapping PCR method.Phylogenetic analysis suggested that Gloverin,Attacin,Moricin proteins ofE.obliqua and E.grisescens were classified into an independem branch using a Neighbor-joining method,respectively.Further determination by qRT-PCR showed that the transcript levels ofgloverin,attacin and moricin genes were up-regulated in the two sibling species after infection with EoNPV.The highest expression level ofgloverin was detected at 12 h group,while moricin was expressed highest at 48 h group.The transcript levels ofgloverin and moricin were significantly higher in E.grisescens than in E.obliqua.However,the transcript levels of attacin were changed at the different time of EoNPV infection between two sibling species,which indicates that gloverin,moricin and attacin are potentially associated with active resistance to virus infection.These results are of significance for further exploring the role of antimicrobial peptide genes in the interaction between insects and viruses.关键词
灰茶尺蛾/小茶尺蠖/EoNPV/抗菌肽Key words
Ectropis grisescens/Ectropis obliqua/EoNPV/antimicrobial peptide分类
农业科技引用本文复制引用
毛腾飞,付建玉,孙亮,周孝贵,白家赫,肖强..两近缘种茶尺蠖的抗菌肽基因在抵抗EoNPV感染过程中的表达差异[J].中国生物防治学报,2017,33(4):472-480,9.基金项目
国家科技支撑计划(2011BAD01B02) （2011BAD01B02）
中国农业科学院科技创新工程(CAAS-ASTIP-2016-TRICAAS) （CAAS-ASTIP-2016-TRICAAS）
