中国生物防治学报2017，Vol.33Issue(4)：487-495,9.DOI:10.16409/j.cnki.2095-039x.2017.04.008
DNA条形码技术在小花蝽属昆虫分类鉴定中的应用
Application of DNA Barcoding Technique for Species Identification of Orius Wolff (Heteroptera: Anthocoridae)
摘要
Abstract
Thirteen Orius species were differentiated using a DNA barcoding technique.Base composition of 59 COI gene fragments were analyzed,intra-and inter-species genetic distances were calculated and phylogenetic trees were established based on neighbor-joining method,maximum parsimony method and bayesian method,respectively.Results showed that base composition of 13 Orius species' COI gene sequence was consistent with the typical insect mitochondrial DNA,A+T average content (66.4 ％) was higher than G+C average content,A+T content was 90.7％ in 3rd position of codon,most base substitutions were synonymous substitutions.Average intra-specific genetic distance was 0.008,average inter-specific genetic distance was 0.128,the two were not overlapped.The results of cluster analysis,based on three different methods,were basically consistent with the results of morphological identification,different individuals of the same population were gathered to a branch in the dendrograms,but there may be a cryptic species ofOrius minutus (Linnaeus).The DNA barcoding technique can be potentially used for quick identification of Orius species.关键词
小花蝽属/DNA条形码技术/COI基因/遗传距离/系统发育树Key words
Orius/DNA barcoding technique/COI gene/genetic distance/phylogenetic tree分类
农业科技引用本文复制引用
胡泽章,孙猛,吕兵,段敏,翟一凡,陈浩,郑礼,于毅..DNA条形码技术在小花蝽属昆虫分类鉴定中的应用[J].中国生物防治学报,2017,33(4):487-495,9.基金项目
国家国际科技合作专项(2013DFA32070) （2013DFA32070）
山东省自然科学基金(ZR2016CB50) （ZR2016CB50）
山东省农业重大应用技术创新专项重点项目 （）
山东省农业科学院学科带头人科研启动项目 （）
