中国生物防治学报2017，Vol.33Issue(4)：561-570,10.DOI:10.16409/j.cnki.2095-039x.2017.04.018
解淀粉芽胞杆菌JDF-6液态发酵条件优化及抑菌蛋白的分析
Optimizing Fermentation Condition for Bacillus amyloliquefaciens JDF-6 and Analysis of Antifungal Proteins
摘要
Abstract
To investigate the best condition for liquid fermentation of Bacillus amyloliquefaciens JDF-6 and the characteristics of antifungal proteins,the liquid fermentation medium and fermentation conditions were optimized by the single factor experiments and orthogonal experiments;crude proteins were extracted by using ammonium sulphate precipitation,and the protease stability,light stability and thermal stability were determined.Results indicated that the optimum medium for Bacillus amyloliquefaciens JDF-6 was 3.0％ sucrose,1％ yeast extract powder,2.0％ oil-tea-cake,0.3％ MnSO4.The optimal fermentation conditions were fermentation time for120 h,temperature at 30 ℃,pH at 6.5-7.0,5％ inoculation quantity,with 10 mL/250 mL medium capacity.Inhibitory activity of crude proteins from strain JDF-6 increased 51.6％,under the optimum fermentation medium and culture conditions.Crude proteins which extracted from strain JDF-6 by using 50％ ammonium sulphate for precipitation were sensitive to proteinase,high temperature (＞ 100 ℃) and UV light (＞ 8 h),with inhibitory activity decreased.关键词
解淀粉芽胞杆菌/抑菌蛋白粗提液/发酵/优化/稳定性Key words
Bacillus amyloliquefaciens/crude proteins/fermentation/optimize/stability分类
农业科技引用本文复制引用
胡飞,胡本进,周子燕,徐丽娜,苏卫华,苏贤岩..解淀粉芽胞杆菌JDF-6液态发酵条件优化及抑菌蛋白的分析[J].中国生物防治学报,2017,33(4):561-570,10.基金项目
安徽省农业科学院院长青年创新基金项目(16B1121) （16B1121）
安徽稻麦(油)轮作区水稻化肥农药减施增效技术集成与示范(2016YFD0200806) （油）
