中国兽医科学2017，Vol.38Issue(8)：939-944,6.DOI:10.16656/j.issn.1673-4696.2017.08.001
口蹄疫病毒结构蛋白VP1与细胞间黏附分子ICAM-2重组融合蛋白的表达及免疫原性分析
Expression and immunogenicity of recombinant fusion protein comprising foot-and-mouth disease virus structural protein VP1 and intercellular adhesion molecule ICAM-2
摘要
Abstract
The gene sequence VP1-ICAM-2 was synthesized by using PAS(PCR-based accurate synthesis) method.The recombinant plasmid was obtained by introducing the synthetic gene VP1-ICAM-2 into the transfer vector pFast-Baclby using BamH Ⅰ and HindⅢ,and then the recombinant plasmidpFast-Bacl-VP1-ICAM-2 was transformed into DH10Bac competent cells for blue-white screening to obtain positive recombinant granules rBacmid-VPl-ICAM-2.The recombinant baculovirus rBac-VPl-ICAM-2 was obtained by transfection of positive recombinant granulocytes into Sf9 cells.The Western-blot showed that the recombinant protein was about 57 ku in size and could be recognized by A-type FMDV positive sera.The guinea pigs experiment showed that the fusion protein VP1-ICAM-2 induced FMDV specific humoral immune and cellular immune responses.This study result provides informatinon for the development of new FMD subunit vaccines.关键词
口蹄疫病毒/融合蛋白/杆状病毒/表达/免疫原性分析Key words
foot-and-mouth disease virus/fusion protein/baculovirus/expression/immunogenicity analysis分类
农业科技引用本文复制引用
芦延珍,刘新生,周鹏,方玉珍,张永光,刘霞,王永录,杜晓华..口蹄疫病毒结构蛋白VP1与细胞间黏附分子ICAM-2重组融合蛋白的表达及免疫原性分析[J].中国兽医科学,2017,38(8):939-944,6.基金项目
国家生猪产业体系项目(CARS-36-068) （CARS-36-068）
“十三五”国家重点研发计划(2016YFD0501505) （2016YFD0501505）
中国农业科学院兰州兽医研究所统筹项目(Y2016CG23) （Y2016CG23）
