中国兽医科学2017，Vol.38Issue(8)：951-956,6.DOI:10.16656/j.issn.1673-4696.2017.08.003
牛传染性鼻气管炎病毒nano-PCR与LAMP及PCR检测方法的对比研究
Comparison of nano-PCR, loop-mediated isothermal amplification and conventional PCR in the detection of bovine infectious laryngotracheitis virus
摘要
Abstract
The purpose of this study was to compare three assays with respect to their ability to accurately detect BoHV-1 in biological samples,namely nano PCR,loop-mediated isothermalamplification (LAMP),and conventional PCR.All assays detected BoHV-1 nucleic acids and differentiated between BoHV-1-free and-contaminated bovine sera successfully.A rapid,specific,sensitive,and practical method of detecting BoHV-1 in bio-products is needed.In addition,the results were specific to BoHV-1:theamplification of samples containing the other pathogenic bovine viruses yielded negative results.The lowest detection threshold,2.23 × 102 copies/μL,was achieved by the nano-PCR assay.This assay was also the most effective in the detection of BoHV-1 in biological samples.The fastest detection was in 50 min,which was achieved by the LAMP assay.The field conditions suggest that nano-PCR is specific and sensitive to detect BoHV-1 in biological samples and may be used for quality control of biomaterials.关键词
牛传染性鼻气管炎病毒/nano-PCR/环介导等温扩增技术/PCRKey words
infectious bovine rhinotracheitis virus/nano-PCR/loop-mediated isothermal amplification/PCR分类
农业科技引用本文复制引用
郭利,李建友,李家伟,王楠,程世鹏..牛传染性鼻气管炎病毒nano-PCR与LAMP及PCR检测方法的对比研究[J].中国兽医科学,2017,38(8):951-956,6.基金项目
吉林省科技厅项目(20150307024NY) （20150307024NY）
“十三五”国家重点研发计划项目(2016YFD0500907) （2016YFD0500907）
吉林省科技厅项目(20170309002NY) （20170309002NY）
