中国兽医科学2017，Vol.38Issue(8)：957-963,7.DOI:10.16656/j.issn.1673-4696.2017.08.004
猪流行性腹泻病毒流行毒株的分离与鉴定
Isolation and identification of a variant porcine epidemic diarrhea virus
摘要
Abstract
A diarrhea outbreak characterized by watery diarrhea and high mortality among neonates occurred in a pig farm in Jiangsu province in March 2016.To identify the pathogen that caused this diarrheal disease,six feces samples were collected and subjected to RT-PCR analysis.The results showed six samples were positive for PEDV,while all of them were negative for TGEV and PoRV.Then all the positive samples were processed and inoculated into the monolayers of Veto cells for virus isolation.Only one sample designated JSqd3/2016 could induce visible cytopathogenic effect(CPE)and the virus isolated from this sample was serially passaged to the 5th generation (JSqd3/2016-FS).After that,the Vero cells inoculated with JSqd3/2016 were tested by indirect immunofluorescence assay(IFA)and the result showed it could be specifically recognized by the monoclonal antibody(MAb)against the PEDV spike(S) protein.Also,the S gene and ORF3 gene of JSqd3/2016-F5 were amplified,cloned,sequenced and used for sequence analysis.The result showed JSqd3/2016 was more closely related to the currently circulating variant PEDV strains;its S gene had pronounced mutations compared with the classical strains and the deduced amino acid sequence similarity between S protein of JSqd3/2016-F5 and the circulating variant PEDV strains ranged from 93.0％to 93.7％.These results suggested JSqd3/2016 was a variant PEDV strain and it was the causative agent of this diarrheal outbreak.关键词
猪流行性腹泻病毒/JSqd3/2016/分离/鉴定Key words
porcine epidemic diarrhea virus/JSqd3/2016/isolation and identification分类
农业科技引用本文复制引用
周书亭,谢洋洋,丁思嘉,郑煜名,蔡雨豪,罗依然,袁聪俐,杨志彪..猪流行性腹泻病毒流行毒株的分离与鉴定[J].中国兽医科学,2017,38(8):957-963,7.基金项目
国家自然科学基金项目(31472211),国家重点研发计划项目(2016YFD0500100),上海市自然基金项目(14ZR1421400) （31472211）
