中国兽医科学2017，Vol.38Issue(8)：964-968,5.DOI:10.16656/j.issn.1673-4696.2017.08.005
环形泰勒虫感染对单核细胞中NOD样受体信号通路相关基因转录的影响
Theileria annulata-infected effection of the transcription levels of 10 genes associated with NOD-like receptors signal pathway
摘要
Abstract
To detect 10 genes(NOD1,NOD2,NLRP1,NLRP3,NLRC4,NAIP,RIP2,CASPI,IL-18 and IL-1β) in bovine NOD-like receptor signal pathway and quantify those gene's transcription levels in the F10 TaNM cells infected with Theileria annulata,the specifical primers of those genes were designed according the sequences which were reported in GenBank.The reaction conditions of real-time PCRs were optimized.The specificity,sensitivity and repeatability of the i0 real-time quantitative PCRs were evaluatied.The results showed that the i0 real-time PCR methods had a signal peak in a dissociation curve,respectivelyed,and their sensitivity were from 1 to i00 copies/μL.It also indicated that those methods were good in specificity and sensitivity.The transcription levels of NOD1,NOD2,NLRP1,NLRP3,NLRC4,NAIP,RIP2,CASP1 and IL-18 in the F10 TaNM cells were lower than that of control group(peripheral blood lymphocytes) NAIP(P＜0.01),but the transcription levels of IL-1βwas sensitively higher than that of control group(P＜0.01).This study laid the foundation for further researches about effection of host immunity function by Theileria annulata infection.关键词
NLRs/real-time PCR/环形泰勒虫/转录水平Key words
NLRs/real-time PCR/Theileria annulata/transcription levels分类
农业科技引用本文复制引用
李四通,罗建勋,关贵全,刘军龙,刘爱红,李有全,赵洪喜,赵帅阳,何欣,陈银银,殷宏..环形泰勒虫感染对单核细胞中NOD样受体信号通路相关基因转录的影响[J].中国兽医科学,2017,38(8):964-968,5.基金项目
国家重点基础研究发展计划(973)项目(2015CB150300) （973）
国家自然科学基金项目(31402189、31072130、31372432) （31402189、31072130、31372432）
农业科技创新工程(ASTIP)国家肉牛牦牛产业技术体系项目(NBCIS CARS-38) （ASTIP）
