中国兽医科学2017，Vol.38Issue(8)：975-980,6.DOI:10.16656/j.issn.1673-4696.2017.08.007
鼠伤寒沙门菌超氧化物歧化酶SodA基因的表达及其酶活性的测定
Expression of Salmonella typhimurium SodA gene and determination of activities of the recombinant SodA
摘要
Abstract
To investigate the biological functions of superoxide dismutase SodA of Salmonella typhimurium,superoxide dismutase SodA gene was cloned from Salmonella typhimurium and expressed in E.coli.The length of the SodA gene was 621 bp,and the molecular weight of the protein was 23.7 ku according to SDS-PAGE.Optimal temperature and pH for the enzymatic activities of SodA were 40℃and 7.0,respectively.The enzymatic activity of SodA was elevated by Mn2+,Zn2+and Cu2+,and inhibited by SDS,chloroformethanol,and Fe3+,but not affected by the addition of H202,Mg2+,Ca2+,Ba2+,Na+and K+.The kinetic parameters vmaxand κm in pyrogallol were 2.03 U/min and 0.801mmol/L,respectively.Moreover,the capacity of SodA were strengthened in acid and alkali-resistance and ethanol-tolerance in the presence of the catalase CAT.The above-mentioned results provided a solid basis for researches concerning the functions of SodA in Salmonella typhimurium in future.关键词
鼠伤寒沙门菌/超氧化物歧化酶/克隆表达/酶学性质Key words
Salmonella typhimurium/superoxide dismutase/cloning and expression/enzymatic activities分类
农业科技引用本文复制引用
张建康,汪洋,易力,孙理云,温文彦,刘一尘,李小康,丁轲,张春杰..鼠伤寒沙门菌超氧化物歧化酶SodA基因的表达及其酶活性的测定[J].中国兽医科学,2017,38(8):975-980,6.基金项目
国家科技支撑计划项目(31540095) （31540095）
家畜疫病病原生物学国家重点实验室(中国农业科学院兰州兽医研究所)开放课题(SKLVEB2013KFKT003) （中国农业科学院兰州兽医研究所）
河南省高校创新人才基金项目(14HASTIT024) （14HASTIT024）
河南省自然科学基金项目(162300410067) （162300410067）
