中国兽医科学2017，Vol.38Issue(8)：981-987,7.DOI:10.16656/j.issn.1673-4696.2017.08.008
副猪嗜血杆菌聚胺转运蛋白PotD重组亚单位疫苗的制备及其免疫保护效力的评价
Development of a Haemophilus parasuis polyamine transport protein PotD subunit vaccine and evaluation of its immune efficacy
摘要
Abstract
To evaluate the immunoprotective efficacy of Haemophilus parasuis polyamvne transport protein potD,the gene without signal peptide coding sequence was amplified from SC1401 genome DNA and subsquently subcloned and expressed in E.coli pET28a(+) and BL21(DE3),respectively.The expression form of recombined PotD was confirmed by ultrasonication,followed by SDS-PAGE.The mice in vaccination group was immunized with 50 μ g purified product emulsified with Freund's complete and incomplete adjuvant(FCA and FIA,respectively)every two weeks,which was fol]owed by antibody titer determination (ELISA) and mice challenge experiment,The results demonstrated that the recombined protein was nearly 44.3 ku,mainly in a form of inclusion bodies.The antibody titer in recombined protein group was significantly higher compared with the formalin-killed vaccine group(P＜0.05)under the dilution of 1∶20 000 (with the P/N＞2.1),and both the vaccine groups of recombined PotD and formalin-killed H.parasuis had a protective rate of 80％.Taken together,our studies demonstrate that the recombined PotD can actively induce immune responses in mice and to protect them in challenge experiment,which can be served as a candidate subunit vaccine in protection of virulent H.parasuis.关键词
副猪嗜血杆菌/PotD蛋白/亚单位疫苗/免疫保护力/聚胺Key words
Haemophilus parasuis/PotD/subunit vaccine/immune efficacy/polyamine分类
农业科技引用本文复制引用
代科,文翼平,谨瑾,文心田,张禄滑,李英,曹三杰,黄小波,伍锐,赵勤..副猪嗜血杆菌聚胺转运蛋白PotD重组亚单位疫苗的制备及其免疫保护效力的评价[J].中国兽医科学,2017,38(8):981-987,7.基金项目
公益性行业(农业)科研专项(201303034) （农业）
