中国兽医科学2017，Vol.38Issue(8)：988-992,5.DOI:10.16656/j.issn.1673-4696.2017.08.009
针对境外口蹄疫疫情的疫苗储备毒株的构建和生物学特性分析
Construction and biological characteristics of reserve vaccine strains of foot-and-mouth disease virus referring to the epidemic situation in neighbor countries
摘要
Abstract
To prevent the risk of trans-boundary transmission of new genotypic FMDV 0/Panasia Ⅱ and A/Iran05 circulating in Asia regions and posing great menace to our national livestock husbandry.In this study,two chimeric viruses were constructed by replacing the whole P1 capsid protein coding region of O/HN/93 and A/GDMM/2013 infectious clones with the corresponding part of 0/Panasia Ⅱ and A/I-ran05,respectively.The recombinant viruses were proved to be rescued and competent for replication by indirect immunofluorescence,RT-PCR,gene sequencing,electronic microscopy,plaque phenotype and one-step growth curves.However,the recombinant viruses showed relatively lower ability of replication and longer time to reach complete CPE compared with parental viruses,being almost 19 hours.These recombinant FMDVs provide very useful materials for studying the vaccine strains against O/Panasia Ⅱ and A/Iran05 incursion.关键词
口蹄疫病毒/P1基因/构建/生物学特性分析Key words
FMDV/P1 gene/construction/biological characteristics analysis分类
农业科技引用本文复制引用
寻广谨,曹轶梅,付元芳,张婧,刘在新,李平花,孙普,马雪青,白兴文,卢曾军,陈应理,包慧芳,李冬..针对境外口蹄疫疫情的疫苗储备毒株的构建和生物学特性分析[J].中国兽医科学,2017,38(8):988-992,5.基金项目
甘肃省农业生物技术研究与应用开发项目(GNSW-2014-22) （GNSW-2014-22）
