中国兽医科学2017，Vol.38Issue(8)：1005-1010,6.DOI:10.16656/j.issn.1673-4696.2017.08.012
细粒棘球绦虫原头蚴对小鼠巨噬细胞RAW264.7一氧化氮分泌的影响
Effects of protoscolices of Echinococcus granulosus on in vitro production of nitric oxide from murine macrophage RAW264.7
摘要
Abstract
Murine macrophages RAW264.7 were cultured on 24-well plates,and were stimulated with the protoscoleces(PSC),hydatid cyst wall(HC) and hydatid cyst fluid(HF) of Echinococcus granulosus.Meanwhile,the PBS group was designed as negative control.The concentration of nitric oxide(NO)was detected by Griess reagent at different time points.The results showed that NO was increased at 8 hours,24 hours and 48 hours post-stimulation(PC) in PSC group,respectively,and there were significant differences compared with that of the PBS control group(P＜0.05).HC also stimulated the NO production by induced macrophages,and the results showed that there were significantly differences compared with that of the PBS control group at 24 hours and 48 hours PC(P＜0.05).HF induced a small quantity of NO at 4 hours,24 hours and 48 hours PC but there were not significantly differences compared with that of the PBS control group(P＞0.05).The results indicated that the macrophages could resist the infection of protoscolices by producing N0.The hydatid cyst wall has a damaging effect on the host,but also can weaken the harmful effects to the protoscolex by reducing the production of NO.The effect of hydatid cyst fluid was not significant to NO production by macrophages.The experiment will be useful for further study on the mechanism of NO against parasite infection.关键词
细粒棘球绦虫/棘球蚴病/原头蚴/一氧化氮/原头蚴/包囊/包囊液/免疫逃避Key words
Echinococcus granulosus/hydatid disease/nitric oxide/protoscolex/hydatid cysts/hydatid cyst fluid/immune escape分类
农业科技引用本文复制引用
卢平萍,王正荣,张艳艳,王智欣,马勋,薄新文..细粒棘球绦虫原头蚴对小鼠巨噬细胞RAW264.7一氧化氮分泌的影响[J].中国兽医科学,2017,38(8):1005-1010,6.基金项目
国家重点基础研究发展计划(973)项目(2015CB150300) （973）
国家自然科学基金项目(31360608) （31360608）
新疆生产建设兵团科技攻关项目(2014BA002) （2014BA002）
