中国现代医学杂志2017，Vol.27Issue(18)：7-14,8.DOI:10.3969/j.issn.1005-8982.2017.18.002
二烯丙基二硫在RORα抑制人胃癌细胞上皮间质转化中的作用
Up-regulation of RORα inhibits EMT in human gastric MGC803 cells induced by diallyl disulfide
摘要
Abstract
Objective To investigate whether diallyl disulfide (DADS) inhibits epithelial mesenchymal trasition (EMT) in human gastric cancer MGC803 cells by up-regulation of retinoid acid receptor related orphan receptor α (RORα).Methods The morphological effect of MGC803 cells was observed by phase contrast microscope.The expressions of molecules correlated to EMT were detected by RT-PCR,Western blot,immunofluorescence and immunohistochemistry.The influence of DADS and silencing RORα on the growth of xenograft tumor in athymic mice was observed.Results Phase contrast microscopy showed that MGC803 cells of RORα silence were discordancy in size and shape and the number of spindle cells increased.Besides,the MGC803 cells treated by DADS were found with characteristics such as size unification,round or ellipse shape,decreased spindle cells and less atypia.RT-PCR and Western blot revealed up-regulation of RORα mRNA and protein in each group treated by DADS (P ＜ 0.05).Moreover,the effect in control group and the vector group increased compared to the RORα silence group treated by DADS (P ＜ 0.05).RT-PCR and Western blot showed the up-regulation of Snail protein and Vimentin mRNA and protein,and down-regulation of E-cadherin mRNA and protein in the RORα silence cells (P ＜ 0.05).However,in the MGC803 cells treated by DADS Snail and Vimentin were down-regulated and E-cadherin was up-regulated (P ＜ 0.05).Immunofluorescence showed that the positive expressions of Snail and Vimentin in the RORα silence cells were strengthened,but E-cadherin was attenuated.Nevertheless,the positive expressions of Snail and Vimentin were attenuated,and E-cadherin was strengthened in the MGC803 cells treated by DADS.The growth of the xenograft tumor was accelerated,and the weight of the transplantation tumor increased in the RORα silence group compred to the control group (P＜ 0.05).The positive expressions of Ki-67,Snail,CD34 and Vimentin were obviously increased,while the positive rate of E-cadherin also increased in the RORα silence group.Inversely,the rate of the xenograft tumor growth slowed down and the volume of tumor was significantly diminished.The expressions of Ki-67,Snail,CD34 and Vimentin decreased and the positive expression of Ecadherin increased in the MGC803 cells treated by DADS.Conclusions DADS can inhibit EMT in MGC803 cells in vivo and in vitro through up-regulation of RORα.关键词
二烯丙基二硫/维甲酸相关孤核受体α/人胃癌MGC803细胞/上皮-间质转化Key words
diallyl disulfide/RORα/human gastric cancer MGC803 cell/EMT分类
医药卫生引用本文复制引用
刘芳,苏坚,曾颖,夏红,苏波,凌晖,曾希,苏琦..二烯丙基二硫在RORα抑制人胃癌细胞上皮间质转化中的作用[J].中国现代医学杂志,2017,27(18):7-14,8.基金项目
国家自然科学基金(No:81374013) （No:81374013）
湖南省卫计委资助项目(No:B2015-182) （No:B2015-182）
湖南省教育厅资助项目(No:17K081) （No:17K081）
