中国现代医学杂志2017，Vol.27Issue(18)：38-42,5.DOI:10.3969/j.issn.1005-8982.2017.18.007
IL-2及IL-2Rα基因多态性与汉族儿童支气管哮喘的关联
Association of IL-2 and IL-2Rα polymorphisms with bronchial asthma in Chinese Han children
刘冬松 1刘安兵 2陈岳明2
作者信息
- 1. 浙江省金华市中医医院检验科,浙江金华321017
- 2. 浙江省杭州市第一人民医院检验科,浙江杭州310006
- 折叠
摘要
Abstract
Objective To investigate the relationship between IL-2 (rs6822844) and IL-2Rα (rs2104286) gene Polymorphisms and bronchial asthma in Chinese Han children.Methods A gender and age-matched case control study was conducted.A total of 144 children with bronchial asthma were enrolled into the case group and 228 healthy children into the control group randomly.The genotypes were determined by direct sequencing for the products of fluorescent quantitative polymerase chain reaction (PCR).The relative expression of IL-2Rα mRNA in the peripheral blood was analyzed by real-time fluorescent PCR.The expression levels of plasma sIL-2Roα were detected by enzyme-linked immunosorbent assay (ELISA) for the different genotype carriers in the control group.Results The allele A distribution frequency of IL-2Rα (rs2104286) in the case group (86.8％) was significantly higher than that of the control group (79.4％) [(O)R=1.708 (95％CI:1.113,2.629),P=0.010].The AA genotype frequency distribution in the case group (75.0％)was higher than that of the control group (62.3％) [(O)R=1.745 (95％ CI:1.058,2.884),P =0.021].The polymorphism distribution of IL-2 (rs6822844) was not found in the study.Additionally,both relative expression of IL-2Roα mRNA in the peripheral blood and the plasma sIL-2Roα level of the individuals with AA genotype were significantly higher than those with AG genotype in the control group (P=0.043 and 0.018,respectively).Conclusions The gene polymorphism of IL-2Rα (rs2104286) may be associated with the occurrence of bronchial asthma in Chinese Han children,and may regulate gene transcription and expression.关键词
支气管哮喘/基因多态性/白细胞介素2/白细胞介素2a受体Key words
bronchial asthma/gene polymorphism/interleukin-2/interleukin-2 receptor alpha分类
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刘冬松,刘安兵,陈岳明..IL-2及IL-2Rα基因多态性与汉族儿童支气管哮喘的关联[J].中国现代医学杂志,2017,27(18):38-42,5.
