中国药理学与毒理学杂志2017，Vol.31Issue(6)：541-546,6.DOI:10.3867/j.issn.1000-3002.2017.06.007
黄芩素对2,4,6-三硝基苯磺酸诱导的小鼠实验性肠炎的作用和机制
Effect and mechanism of baicalein on 2,4,6-trinitrobenzene sulfonic acid-induced experimental colitis of mice
摘要
Abstract
OBJECTIVE To explore the effect and mechanisms of baicalein on 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis in mice.METHODS BALB/c mice were randomly placed into three groups (n=10):normal control group,TNBS group,and TNBS+baicalein (20 mg· kg-1,once per day) group.Mouse colitis was induced by intrarectal injection of TNBS.Baicalein was administered by oral gavage two days prior to TNBS treatment and until the end of the study (a total of 9 d).The colon length was measured before HE staining was performed for histological damage assessment.The remaining colon pieces were collected to measure the content of tumor necrosis factor-α(TNF-α).Lipopolysaccharide (LPS)-stimulated RAW264.7 mouse macrophage was used as a cell model to determine the content of nitric oxide (NO) in cell culture medium,the mRNA levels of TNF-α,interleukin-6(IL-6),IL-1β,inducible nitric oxide synthase(iNOS),cyclooxygenase 2(COX-2) and monocyte chemoattractant protein-1 (MCP-1),and the protein expression of phosphatidylinositol 3-kinase/protein kinase B/nuclear factor-κB (PI3K/AKT/NF-κB) pathway.RESULTS Baicalein significantly attenuated TNBS-induced colon shortening and histological injury (P＜0.05),which was correlated with the decline in the content of TNF-α in the colon.According to the jn vivo results,baicalein exposure down-regulated the secretion of NO and the mRNA expression of pro-inflammatory mediators (iNOS,COX-2,MCP-1,TNF-α,IL-1β and IL-6) in LPS-stimulated RAW264.7 cells (P＜0.05,P＜0.01).Additionally,the phosphorylation/activation of LPS-stimulated PI3K/AKT/NF-κB pathway was inhibited by baicalein treatment.CONCLUSION The beneficial effect of baicalein in TNBS-induced experimental colitis may be due to PI3K/AKT/NF-κB signaling inhibition.关键词
肠炎/磷脂酰肌醇3-激酶/蛋白激酶B/NF-κB/黄芩素Key words
colitis/phosphatidylinositol 3-kinase/protein kinase B/NF-κB/baicalein分类
医药卫生引用本文复制引用
罗晓平,于之伦,邓超,张晶晶,王峥涛,窦薇..黄芩素对2,4,6-三硝基苯磺酸诱导的小鼠实验性肠炎的作用和机制[J].中国药理学与毒理学杂志,2017,31(6):541-546,6.基金项目
国家自然科学基金(81273572) （81273572）
国家自然科学基金(81530069)The project supported by National Natural Science Foundation of China(81273572) （81530069）
and National Natural Science Foundation of China(81530069) （81530069）
