中国药理学与毒理学杂志2017，Vol.31Issue(6)：568-573,6.DOI:10.3867/j.issn.1000-3002.2017.06.011
雄激素对雌激素引发去势雄性SD大鼠前列腺炎症及炎症因子的抑制作用
Inhibitory effect of testosterone on prostatitis and inflammatory factors of castrated male SD rats initiated by estrogen
摘要
Abstract
OBJECTIVE To study the changes in inflammatory factors caused by prostatitis.METHODS SD rats were castrated under sterile conditions.E2 0.25 mg· kg-1+ T 0.25,0.5 and 1.0 mg· kg-1 were given sc for 30 d,respectively.Serum samples were taken and levels of E2,T and dihydrotestosterone (DHT) were detected by ELISA.Pathological changes of prostate tissue were observed by HE staining.The expressions of tumor necrosis factor-alpha (TNF-α),cyclooxygenase-2 (COX-2) and macrophage inflammatory protein 1alpha (MIP-1α) in prostate were detected by immunohistochemistry.RESLULTS ELISA detection showed that E2 levels were significantly increased [(80±7) ng· L-1,P＜0.01] in E2 0.25 mg· kg-1 group and that T levels were significantly decreased [111 ±6 vs (111 ±5) nmol· L-1,P＜0.05]in E20.25 mg ·kg-1 and E2+T 0.25 mg·kg-1 groups compared with the sham-operated group.E2 was significantly increased [(80±7) ng· L-1,P＜0.01] in E20.25 mg· kg-1 groups compared with the castrated control.The sham and castrated control showed normal glandular epithelium without leukocyte infiltration.In E2 0.25 mg·kg-1 group,extensive infiltration of inflammatory cells was found in the glandular lumens,suggesting the occurrence of chronic prostatitis.In each E2+T groups,fewer inflammatory cells were noted in the stroma around glands.The expressions of TNF-m COX-2 and MIP-1α in sham group were negative or low,while those of castrated control and E2 0.25 mg· kg-1 groups were high,especially in E2 0.25 mg· kg-1 group.The expressions of TNF-α,COX-2 and MIP-1α in each E2+T group were significantly decreased.CONCLUSION Testosterone can inhibit prostatitis and the expression of inflammatory factors,such as TNF-α,COX-2 and MIP-1 α,in castrated SD rats initiated by estrogen.关键词
前列腺炎/雌激素/雄激素Key words
prostatitis/estrogen/testosterone分类
医药卫生引用本文复制引用
贾玉玲,崇立明,李雷,马爱翠,陈颖,周莉,孙祖越..雄激素对雌激素引发去势雄性SD大鼠前列腺炎症及炎症因子的抑制作用[J].中国药理学与毒理学杂志,2017,31(6):568-573,6.基金项目
上海市研发平台专项(15DZ2290400) （15DZ2290400）
上海市“科技创新行动计划”实验动物研究项目(14140901302)The project supported by Shanghai Research and Development Platform (15DZ2290400) （14140901302）
and Shanghai "Innovative Action Plan" Experimental Animal Study (14140901302) （14140901302）
