中国药理学与毒理学杂志2017，Vol.31Issue(6)：621-625,5.DOI:10.3867/j.issn.1000-3002.2017.06.018
孕期尼古丁暴露致成年雄性仔鼠心肌纤维化
Prenatal nicotine exposure induces cardiac fibrosis in adult male offspring
摘要
Abstract
OBJECTIVE To investigate the effect of prenatal nicotine exposure on cardiac ejection function and myocardial fibrosis of the offspring of rats.METHODS Pregnant rats were sc given nicotine 6.0 mg· kg-1,once daily for 17 d.The body mass and heart mass of the offspring were detected at the 21th day of gestation,and 15 and 90 d after birth.Heart rate of 90 d offspring was recorded by ECG,and cardiac functions were detected by Doppler ultrasonography,including cardiac output (CO),stroke volume (SV),ejection fraction (EF),left ventricular long axis shortening fraction (FS),interventricular septum diastolic diameter (IVSd) and left ventricular posterior wall diastolic diameter (LVPWd).The myocardial ultrastructure was detected under an electron microscope.Masson staining was used to detect the myocardial collagen fiber deposition.The level of collagen protein type Ⅰ in heart tissue was detected by radioimmunoassay.RESULTS Compared with control group,prenatal nicotine exposure resulted in a decrease of heart mass and body mass in groups of 21 d fetal rats and 15 d offspring(P＜0.05,P＜0.01),but had no effect on the 90 d offspring.Compared with the normal control group,the heart rate of 90 d offspring increased [366+10 vs (418+10) min-1] (P＜0.05),CO,FS and EF decreased (P＜0.01),and IVSd and LVPWd increased (P＜0.05,P＜0.01).Electron microscopy revealed that in the heart of nicotine 90 d offspring,myocardial fiber arrangement was loosened and confused,while extracellular matrix increased.Masson staining showed collagen deposited in the myocardium.The level of collagen type Ⅰ in heart tissue increased [0.59±0.09 vs (0.40±0.05) tμg·g-1 tissue] (P＜0.01).CONCLUSION Prenatal nicotine exposure induces the increased level of cardiac collagen type Ⅰ,myocardial fibrosis and decrease of cardiac ejection function in adult offspring,which may lead to increased susceptibility to cardiovascular diseases.关键词
尼古丁/怀孕/心射血功能/心肌纤维化/Ⅰ型胶原蛋白Key words
nicotine/pregnancy/cardiac ejection function/myocardial fibrosis/collagen type Ⅰ分类
医药卫生引用本文复制引用
董伟家,李希宁,夏志恒,张于娟..孕期尼古丁暴露致成年雄性仔鼠心肌纤维化[J].中国药理学与毒理学杂志,2017,31(6):621-625,5.基金项目
国家自然科学基金(81200482) （81200482）
江苏省高校自然科学研究项目(SZ2170311)The project supported by National Natural Science Foundation of China (81200482) （SZ2170311）
and Natural Science Research Project of Colleges and Universitys in Jiangsu Province (SZ2170311) （SZ2170311）
