中国药理学与毒理学杂志2017，Vol.31Issue(6)：689-695,7.DOI:10.3867/j.issn.1000-3002.2017.06.028
毒理学研究的高内涵筛选及其在药物肝毒性研究中的应用
High-content screening in studies of toxicology and its application in drug-induced hepatotoxicity
摘要
Abstract
Drug-induced liver injury(DILI) is one of the major causes of termination of drug development.The establishment of a high-throughput test system to predict potential clinical hepatotoxicity is a valuable approach in the pharmaceutical industry.The high-content imaging-based in vitro assays allow simultaneous detection of cellular multiple parameters in the system.The real-time monitoring of multiple signaling pathways can shed light on many mechanisms of cell injury,with high sensitivity and specificity.Many types of liver cell models have been applied to high-content screening(HCS) so far.This paper introduceds the HCS technology and reviews the data of hepatotoxicity obtained from HCS technology in recent years.At the same time,we discuss the application of this technology in exploring the mechanism of hepatotoxicity and the potential of HCS technology in studying DILI and mechanisms.关键词
高内涵筛选/肝毒性/毒理学Key words
high-content screening/hepatotoxicity/toxicology分类
医药卫生引用本文复制引用
葛帅,汤纳平,付立杰,马璟..毒理学研究的高内涵筛选及其在药物肝毒性研究中的应用[J].中国药理学与毒理学杂志,2017,31(6):689-695,7.基金项目
国家科技部重大专项定向择优项目(2015ZX-09501004-002-006) （2015ZX-09501004-002-006）
国家科技部重大专项定向择优项目(2015ZX09501-007-003)The project supported by Special Grant from Advanced Programs of National Science and Technology Major Project of China (2015ZX09501004-002-006) （2015ZX09501-007-003）
and Advanced Programs of National Science and Technology Major Project of China (2015ZX09501-007-003) （2015ZX09501-007-003）
