中国肿瘤生物治疗杂志2017，Vol.24Issue(8)：815-827,13.DOI:10.3872/j.issn.1007-385x.2017.08.001
基因编辑技术的原理及其在癌症研究中的应用
Principles and applications of genome-editing technologies in cancer research
摘要
Abstract
Genome-editing technologies that enable precise and stable modification of the genome are sparking a revolution in life science research and disease management.The discovery of the CRISPR/Cas9 technology represents a huge breakthrough in the field of genome editing due to its easy operation and high efficiency,empowering scientists to manipulate genome editing to a broader extension.In this review,the classification of genome-editing technologies,the potential applications of the CRISPR/Cas9 system (including gene knockout,knock-in,methods of delivering CRISPR/Cas9 into cells,enhancing specificity of the CRISPR/Cas9,and accurate regulation of the CRISPR/Cas9) as well as broader applications of the CRISPR/Cas9 system (including base replacement,epigenetic regulation,regulation of endogenous gene expression,and genome-wide genetic screening) will be elaborated,respectively.Moreover,CRISPR/Cas9 in the field of cancer research,including generation of mouse models of cancer,generation of cancer models of chromosome rearrangement,high-throughput screening of genes associated with tumor metastasis,as well as treatment of cancer will also be illustrated,providing readers with a general understanding of the CRISPR/Cas9 technology.关键词
基因编辑/CRISPR/Cas9/癌症Key words
genome-editing/CRISPR/Cas9/cancer分类
生物科学引用本文复制引用
谢一方,王永明..基因编辑技术的原理及其在癌症研究中的应用[J].中国肿瘤生物治疗杂志,2017,24(8):815-827,13.基金项目
国家自然科学基金资助项目(No.81370302). Project supported by the National Natural Science Foundation of China (No.81370302) （No.81370302）
