中国肿瘤生物治疗杂志2017，Vol.24Issue(8)：833-837,5.DOI:10.3872/j.issn.1007-385x.2017.08.003
RNA干扰靶向沉默诱骗受体3基因增加人胰腺癌细胞的放射敏感性
siRNA targeting decoy receptor 3 gene increases the radiosensitivity of human pancreatic cancer cells
摘要
Abstract
Objective:To investigate the effect of siRNA targeting decoy receptor 3 gene on the radiosensitivity of pancreatic cancer cells and its related mechanism.Methods:Plasmid stably expressing DcR3 siRNA sequence was constructed and transfected into pancreatic cancer AsPC-1 cell line by liposome;control group,siRNA(-) negative control group and DcR3 siRNA group were set up.Then DcR3 expression in AsPC-1 cells was detected by Western blotting.Effect ofDcR3 siRNA transfection on radiation sensitivity ofAsPC-1 cells was detected by plate clone formation assay.Cell apoptosis was analyzed by Flow cytometry;the expressions ofCaspase-8,Caspase-3 and PARP-1 were detected by Western blotting and RT-PCR after transfecting with DcR3 siRNA.Results:The expression of DcR3 protein in DcR3 siRNA group was significantly lower than that in control or siRNA(-) group (P＜0.01).The colony formation rate ofDcR3 siRNA group was significantly lower than that in control group and siRNA(-) group (P＜0.01),and the survival fraction(SF) value of DcR3 siRNA group was decreased and the ratio of αββ was increased (P＜0.01).The cell apoptosis rate of DcR3 siRNA group was significantly higher than that of control group or siRNA(-) group (P＜0.01).DcR3 siRNA could significantly up-regulate the expression of Caspase-8 and Caspase3 and down-regulate the expression of PARP-1.Conclusion:siRNA silencing DcR3 gene can increase the radiosensitivity of pancreatic cancer cells by activating the apoptosis factors Caspase-8 and Caspase-3,promoting cell apoptosis.关键词
胰腺癌/AsPC-1细胞/诱骗受体3基因/RNA干扰/放射敏感性Key words
pancreatic cancer/AsPC-1 cell/decoy receptor 3 gene (DcR3)/RNA small interference/radiosensitivity分类
医药卫生引用本文复制引用
肖华平,李庆,谢辉,罗春阳,方玉江..RNA干扰靶向沉默诱骗受体3基因增加人胰腺癌细胞的放射敏感性[J].中国肿瘤生物治疗杂志,2017,24(8):833-837,5.基金项目
湖南省自然科学基金资助项目(No.14JJ3136) （No.14JJ3136）
郴州市科技局科研基金资助项目(No.CZ2013096). Project supported by the Natural Science Foundation of Hunan Province(No.14JJ3136),and the Research Fundation of Chenzhou Science and Technology Bureau (No.CZ2013096) （No.CZ2013096）
