中国肿瘤生物治疗杂志2017，Vol.24Issue(8)：838-844,7.DOI:10.3872/j.issn.1007-385x.2017.08.004
MicroRNA-150对NK/T细胞淋巴瘤组织和NK-92细胞辐射敏感性的影响
Effect of microRNA-150 on the radiosensitivity of NK/T cell lymphoma tissues and NK-92 cells
摘要
Abstract
Objective:To investigate the expression of MicroRNA-150 (miR-150) in NK/T cell lymphoma tissues and its effect on radio-sensitivity of NK-92 cells.Methods:Thirty-six patients with NK/T cell lynphoma that treated in Zhujiang Hospital Affiliated to Southern Medical University were included as study subjects,and their tissue samples were collected.All the patients received similar radiotherapy,and the short-term efficacy was evaluated by the standard of International Workgroup (IWG) of Lymphoma.The patients were further divided into CR (complete remission) group and Non-CR (non-complete remission) group according the treatment efficacy.The miR-150 expression in lymphoma tissues and NK/T cell lymphoma cell lines were detected by qRT-PCR.The NK-92 cells were then transfected with miR-150 mimics.The effect of miR-150 mimics on NK-92 cell radiosensitivity was analyzed by MTT and colony formation assay;effect of miR-150 mimics on radiation induced apoptosis of NK-92 cells were analyzed by Flow Cytometry;and the effect of miR-150 mimics on the expression of apoptosis-related proteins (Caspase 3 and PARP) in NK-92 cells was determined by Western blotting.Results:According to IWG criteria,12 patients had CR while the other 24 patients had Non-CR.Compared with CR group,the microRNA-150 level in NonCR group was significantly decreased(P＜0.05).Compared with the normal sCD3-CD56+ NK cells,miR-150 was significantly lower in NK/T cell lymphoma tissues (9.10 ±0.19 vs 4.01± 0.22 P＜0.01) and five NK/T cell lymphoma cell lines (P＜0.05);over-expression of miR-150 significantly decreased the proliferation and colony formation of NK-92 cells (all P＜0.01),and increased the sensitivity enhancement ratio (SER) after radiation (the SER was 5.375 after 10 Gy exposure;in addition,over-expressions of miR-150 significantly promoted the radiation-induced apoptosis of NK-92 cells [(37.3± 1.24)％ vs (28.3±2.34)％,P＜0.05],and promoted the protein expression of caspase 3 and PARP in NK-92 cells.Conclusion:miR-150 expression significantly decreased in both NK/T cell lymphoms tissues and cell lines.The patients of low miR-150 expression had low CR after radiotherapy.miR-150 mimics transfection promoted radiation-induced apoptosis of NK-92 cells.miR-150 has an enhancement effect on radio-sensitivity of NK/T cell lynphoma.关键词
microRNA-150(miR-150)/NK/T细胞淋巴瘤/辐射敏感性/增殖/凋亡Key words
microRNA-150 (miR-150)/NK/T cell lymphoma/radiosensitivity/profiveration/apoptosis分类
医药卫生引用本文复制引用
吴少杰,黄宇贤,陈俊,郭坤元..MicroRNA-150对NK/T细胞淋巴瘤组织和NK-92细胞辐射敏感性的影响[J].中国肿瘤生物治疗杂志,2017,24(8):838-844,7.基金项目
国家自然科学基金青年项目资助(No.81400156).Project supported by the National Natural Science Foundation for Youth Scholar of China(No.81400156) （No.81400156）
