中国肿瘤生物治疗杂志2017，Vol.24Issue(8)：851-855,5.DOI:10.3872/j.issn.1007-385x.2017.08.006
转基因细胞株BaF3-RAE1ε诱导产生的MDSC的杀伤功能及对NK细胞功能的影响
The cytotoxicity of MDSC-derived from BaF3-RAE1ε injected mice and its effect on NK cell function
摘要
Abstract
Objective:To study the cytolytic activity of myeloid-derived suppressor cell (MDSC) from BaF3-RAE1 ε injected mice and its effect on the function of NK cell.Methods:Two derivatives of murine pro-B cell line BaF3 cells,expressing empty plasmid (termed BaF3-mock) or retinoic acid early transcript 1ε (RAE1ε,termed BaF3-RAE1ε) were respectively constructed in the previous study.CD1 1b+Gr-1 +MDSC magnetically purified from BaF3-mock or BaF3-RAE1ε bearing mice were co-cultured with NK cells.After 24 hours,the cells were harvested for detecting NKG2D and CD 107a expression on NK cells by flow cytometry,and the supematants were collected for detecting IFN-γ by ELISA.Moreover,MDSC sorted from BaF3-mock or BaF3-RAElε bearing mice were cocultured with BaF3-mock or BaF3-RAElε target cells.After 5 h,the cytotoxicity of MDSC against BaF3-mock or BaF3-RAE1 target cells was evaluated by the lactate dehydrogenase release test.Results:There was no obvious difference in secretion of IFN-γ by NK cells co-cultured with MDSC from BaF3-mock or BaF3-RAE1ε bearing mice (P＞0.05).No difference in NKG2D and CD107a expression was detected among NK cells co-cultured with MDSC from BaF3-mock or BaF3-RAE1ε bearing mice (P＞0.05).MDSC isolated from BaF3-RAE1ε bearing mice had higher direct cytolytic activity against BaF3-mock or BaF3-RAE 1εcells than MDSC isolated from BaF3-mock bearing mice (P＜0.01).Conclusion:RAE1εenhances the cytolytic activity of MDSC against BaF3-mock or BaF3-RAE1εcells.关键词
视黄酸早期转录因子1ε/髓系抑制性细胞/NK细胞Key words
retinoic acid early transcript 1ε/myeloid-derived suppressor cell/NK cell分类
医药卫生引用本文复制引用
钱莉,丁婧娟,徐雨薇,刘阳,刘露,叶枫,王少卿,贾筱琴,傅奕,龚卫娟,田芳..转基因细胞株BaF3-RAE1ε诱导产生的MDSC的杀伤功能及对NK细胞功能的影响[J].中国肿瘤生物治疗杂志,2017,24(8):851-855,5.基金项目
国家自然科学基金资助项目(No.81373130,No.81001308) （No.81373130,No.81001308）
江苏省自然科学基金资助项目(No.BK2010315) （No.BK2010315）
扬州大学大学生创新创业训练计划项目.Project supported by the National Natural Science Foundation of China (No.81373130,No.81001308),the Natural Science Foundation of Jiangsu Province (No.BK2010315),and the Science and Technology Innovation Foundation for Undergraduates of Yangzhou University （No.81373130,No.81001308）
