中国肿瘤生物治疗杂志2017，Vol.24Issue(8)：856-863,8.DOI:10.3872/j.issn.1007-385x.2017.08.007
MDA-7/IL-24对Burkitt淋巴瘤细胞的诱导分化作用
Differentiation-inducing effect of MDA-7/IL-24 on Burkitt lymphoma cells
摘要
Abstract
Objective:To investigate the differentiation-inducing effect of melanoma differentiation associated gene 7 (MDA-7)/IL-24 on human Burkitt lymphoma cells,as well as its underlying mechanism.Methods:Human Burkitt lymphoma cell lines (Raji and Daudi) that stably over-expressing MDA-7/IL-24 were constructed.The effect ofMDA-7/IL-24 transfection on cell viability of Raji and Daudi cells was assayed by MTS method;the effect on migration and invasion of Raji and Daudi cells were analyzed by Transwell assay.The apoptosis and immunophenotypes of Raji and Daudi cells with MDA-7/IL-24 over-expression were analyzed by flow cytometry.Meanwhile,the expressions of differentiation related proteins (Myb,BLIMP1 and BCL-6) were analyzed by Western blotting.Raji cell xenograft model was established on nude mice to analyze the effect of MDA-7/IL-24 on the bioactivity of Raji cells in vivo.Results:The cell viability,migration and invasion ability of Raji and Daudi cell lines that transfected with MDA-7/IL-24 were obviously decreased (all P＜0.01),however,the apoptosis cells were not increased (P＞0.05).The expressions of CD45 and CD138 were elevated (P＜0.01) while the expression of CD10 was decreased (P＜0.01) in Raji and Daudi cells overexpressing MDA-7/IL-24.MDA-7/IL-24 over-expression in Raji and Daudi cells significantly increased the expression of BLIMP1 (P＜0.01),but decreased the expression of Myb and BCL-6 (P＜0.01).The tumor mass of nude mice was significantly decreased after the treatment of MDA-7/IL-24 ([1.23±0.21] vs [1.96±0.24]g,P＜0.01).Conclusion:Transfection with MDA-7/IL-24 inhibited the bio-actively of Burkitt lymphoma cells,which might be accomplished by inducing differentiation.关键词
黑色素瘤分化相关基因-7/白介素-24/诱导分化/Burkitt淋巴瘤/MybKey words
melanoma differentiation associatedgene 7(MDA-7)/IL-24/differentiation inducing/Burkitt lymphoma/Myb分类
医药卫生引用本文复制引用
马鸣,杨兴肖,赵连梅,王雪晓,张璁,刘丽华,单保恩..MDA-7/IL-24对Burkitt淋巴瘤细胞的诱导分化作用[J].中国肿瘤生物治疗杂志,2017,24(8):856-863,8.基金项目
河北省卫计委指令性课题资助项目(No.20160171).Project supported by the Medical Scientific Research Key Program of Hebei Province (No.20160171) （No.20160171）
