中国肿瘤生物治疗杂志2017，Vol.24Issue(8)：864-869,6.DOI:10.3872/j.issn.1007-385x.2017.08.008
miR-186通过下调垂体瘤转化基因1的表达抑制骨肉瘤细胞的增殖侵袭并诱导其凋亡
miR-186 suppresses the proliferation and invasion and promotes apoptosis of osteosarcoma cells partially through targeting PTTG1
摘要
Abstract
Objective:To explore the effects of miR-186 on the cell proliferation,apoptosis and invasion of human osteosarcoma cells,and identify its putative mechanisms.Methods:The expression of miR-186 in osteosarcoma cell lines (HOS,U2-OS and Saos-2) and osteoblast NHOst cells was detected using RT-PCR assays.Artificially synthesized miR-186 mimic and relative control scramble mimic was transfected into osteosarcoma HOS and U2-OS cell lines,and the expression of miR-186 in OS cells was detected using the RT-PCR assays upon transfection.Then,the effects of miR-186 over-expression on cell proliferation,apoptosis and invasion of OS cells were explored using the CCK-8,FCSE and transwell invasion assays,respectively.The effects of miR-186 over-expression on mRNA and protein expression of PTTG 1 (pituitary ttmor transforming gene 1) were explored using the Westem blotting and RT-PCR assays.Results:miR-186 was low expressed in OS cell lines;Transfection with artificially synthesized miR-186 mimic significantly up-regulated the expression of miR-186 in HOS and U2-OS cells;the proliferation rate of cells transfected with miR-186 mimic was much lower than those transfected with scramble mimic [HOS:(16.9±2.1)％ vs (10.4±1.6)％;U2-OS:(22.6±2.9)％ vs (14.1±2.2)％;(P＜0.01);the apoptotic rates of HOS and U2-OS cells transfected with miR-186 mimic were higher than those transfected with scramble mimic [HOS:(16.9±2.1)％ vs (10.4± 1.6)％;U2-OS:(22.6±2.9)％ vs (14.1 ±2.2)％;all P＜0.05],and the number of cells passing through the chambers in miR-186 mimic transfection group was less than those of scramble transfection group (P＜0.01).The expression levels of PTTG1 at protein and mRNA level were both suppressed in cells transfected with miR-186 mimic (P＜0.01);however,scramble transfection group showed no statistical difference (P＞0.05).Conclusion:Overexpression of miR-186 significantly inhibited cell proliferation and invasion,and promoted the apoptosis of OS cells,which might be related with PTTG1 suppression.关键词
miR-186/骨肉瘤/垂体瘤转化基因1Key words
miR-186/osteosarcoma/pituitary tumor transforming gene 1 (PTTG1)分类
医药卫生引用本文复制引用
李乾,魏志辉,肖前仁,陈佳骏,周新,王滕羽,张中卒,张铭华..miR-186通过下调垂体瘤转化基因1的表达抑制骨肉瘤细胞的增殖侵袭并诱导其凋亡[J].中国肿瘤生物治疗杂志,2017,24(8):864-869,6.基金项目
国家自然科学基金资助项目(No.81502329) （No.81502329）
重庆医科大学附属永川医院院内课题资助项目(No.YCZQN201514).Project supported by the National Natural Science Foundation of China (No.81502329),and the Foundation of the Yongchuan Hospital of Chongqing Medical University Grants (No.YCZQN201514). （No.YCZQN201514）
