植物学报2017，Vol.52Issue(4)：453-464,12.DOI:10.11983/CBB17044
拟南芥金属蛋白酶FtSH4通过生长素与活性氧调控叶片衰老
Arabidopsis Metalloprotease FtSH4 Regulates Leaf Senescence Through Auxin and Reactive Oxygen Species
摘要
Abstract
The plant metalloproteinases FtSH gene family has 12 members in Arabidopsis,and their functions are still unclear.In the present study,we analyzed the function of FtSH4 on leaf senescence using cell biology and genetics methods.The ftsh4-4 mutant displayed a premature leaf senescence phenotype with increased H2O2 content and cell death rate,decreased chlorophyll content,increased peroxidase gene expression and peroxidase activity.The ftsh4-4 leaf senescence phenotype could be rescued by applying the exogenous antioxidant AsA and endogenous or exogenous auxin by decreasing H2O2 content,peroxidase gene expression level and peroxidase activity.The expression of auxin response factor genes ARF2 and ARF7 was increased in the ftsh4-4 mutant and was reduced by exogenous auxin or AsA.Moreover,H2O2 content and the senescence phenotype of ftsh4-4 could be rescued by the arf2-8 mutant.These results indicate that FtSH4 gene plays an important role in the regulation of leaf senescence through auxin and reactive oxygen species.关键词
FtSH4/拟南芥/活性氧/生长素/叶片衰老Key words
FtSH4/Arabidopsis/reactive oxygen species/auxin/leaf senescence引用本文复制引用
张盛春,李清明,阳成伟..拟南芥金属蛋白酶FtSH4通过生长素与活性氧调控叶片衰老[J].植物学报,2017,52(4):453-464,12.基金项目
国家自然科学基金(No.31370350,No.31271471) （No.31370350,No.31271471）
