安徽农业大学学报2017，Vol.44Issue(4)：558-562,5.DOI:10.13610/j.cnki.1672-352x.20170811.001
四球茶转录组SSR位点信息分析
Analysis of SSR information in Camellia tetracocca Zhang transcriptome
摘要
Abstract
In this study,a total of 99 741 unigenes were de novo assembled based on the bud transcriptome sequencing of Camellia tetracocca Zhang.Totally,23 732 SSRs were detected in 18 552 unigenes,accounting for 23.79％ of all the unigenes.The total length of unigenes containing SSR loci was 226 158 bp.The mean distance between SSRs was 1/2.07 kb and the mean length of the repeat motif was 9.53 bp.Di-nucleotide repeats were the most abundant followed by tri-nucleotide repeats,making up about 47.53％ and 25.92％ of the total SSRs,respectively.Among the 181 repeat motifs,AG/CT,A/T and ACC/GGT were the most frequent motifs in di-,mono-and tri-nucleotide repeats,accounted for 41.38％,22.10％ and 6.49％,respectively,and which totally accounted for 69.97％ of the total SSRs.These results will provide abundant sequences for SSR markers development on Camellia tetracocca Chang.关键词
四球茶/转录组/SSRKey words
Camellia tetracocca Zhang/transcriptome/SSR分类
农业科技引用本文复制引用
黎瑞源,邢辉,申铁..四球茶转录组SSR位点信息分析[J].安徽农业大学学报,2017,44(4):558-562,5.基金项目
国家自然科学基金地区项目(31460233)和贵州师范大学博士基金(11904/0514027)共同资助. （31460233）
