安徽农业大学学报2017，Vol.44Issue(4)：567-573,7.DOI:10.13610/j.cnki.1672-352x.20170811.020
利用枯草芽孢杆菌发酵生产γ-聚谷氨酸
Production of poly-γ-glutamic acid by fermentation of Bacillus subtilis
摘要
Abstract
In this study,we optimized the fermentation parameters of poly-γ-glutamic acid produced by Bacillus subtilis,a strain selected through a lab screen.A single factor experiment and PB experiment were first carried out,and then the optimal composition of the medium and optimum culture conditions were obtained by optimizing the fermentation process with Box-Behnken.The yield of γ-PGA increased from 6.03 g·L-1 to 10.98 g·L-1 at the shaker level.We further explored the feeding process to determine the best time and feeding materials.Finally,a 5 L fermentor was used to increase the production,and the highest yield of γ-PGA reached to 31.18 g·L-1.关键词
枯草芽孢杆菌/γ-PGA/发酵工艺优化Key words
Bacillus subtilis/γ-PGA/optimization of fermentation process分类
轻工纺织引用本文复制引用
桑秀梅,王晶,赵杰,高品,刘萍,殷志敏..利用枯草芽孢杆菌发酵生产γ-聚谷氨酸[J].安徽农业大学学报,2017,44(4):567-573,7.基金项目
江苏省科技厅产学研联合创新资金“前瞻性研究项目(BY2013001-03)”资助. （BY2013001-03）
