安徽农业大学学报2017，Vol.44Issue(4)：584-590,7.DOI:10.13610/j.cnki.1672-352x.20170811.017
版纳微型猪近交系AO血型基因外显子7和外显子8遗传特征分析
Genetic analysis of exon 7 and exon 8 of the UDP-GalNAc gene in Banna Mini-pig inbred line
摘要
Abstract
The porcine UDP-N-acetylgalactosamine transferase (UDP-GalNAc) is the enzyme responsible for transfering a molecule of N-acetylgalactosamine (NAcGal) to the H substrate to synthesize A antigen,which determines A group,the blood group A gene encoding A transferase.In this study,we PCR amplified the exon 7 and exon 8 sequences of the UDP-GalNAc gene (134 bp and 341 bp in length,respectively) in Banna Mini-pig inbred line.Sequencing of both the exons yielded just one single genotype in the tested population.Homology search in NCBI database with 14 species revealed that the similarity varied from 100 ％ (pig) to 72.6 ％ (mice).We documented a deletion of six bases (from the 92th to the 97th) in exon7 in Banna Mini-pig when compared to human,which resulted in missing of two amino acids.Phylogenetic analysis with 14 selected species revealed that Banna Mini-pig shared the same genetic cluster with cattle,sheep,goat and minke whale.关键词
UDP-N-乙酰半乳糖胺转移酶基因/版纳微型猪近交系/外显子7和8/PCR/序列分析Key words
UDP-N-acetylgalactosamine transferase gene/Banna Mini-pig Inbred Line/exon 7 and 8/PCR (polymerase chain reaction)/sequence analysis分类
农业科技引用本文复制引用
潘伟荣,查星琴,霍金龙,卿玉波,肖晶,魏红江,曾养志,成文敏..版纳微型猪近交系AO血型基因外显子7和外显子8遗传特征分析[J].安徽农业大学学报,2017,44(4):584-590,7.基金项目
云南省教育厅科学研究基金项目(2014Z077)资助. （2014Z077）
