重庆理工大学学报（自然科学版）2017，Vol.31Issue(8)：113-119,7.DOI:10.3969/j.issn.1674-8425(z).2017.08.019
喀斯特地貌中碳酸酐酶微生物鉴定与特性研究
Identification and Characterization of Bacterium with Carbonic Anhydrase in Karst Landform
摘要
Abstract
Carbonic anhydrase(CA) which can catalyze inter-conversion between CO2 and HCO-3 rapidly exists widely in prokaryote cells, and the unique conditions of Karst landform is very suitable for the expression of microbial carbonic anhydrase.In this paper, the soil samples were collected from the Jinfoshan scenic spot with the typical Karst landform in Chongqing, isolating bacterium with strains high-yielding carbonic anhydrase.A strain with the highest enzyme activity was selected to perform gram stain microscopy and evaluate the stationary phase.Getting the species of the strain by the identification result of 16S rRNA.Then, it explores the influence of the main environmental factors (such as temperature, pH, mental ions and anions), on the activity of it's carbonic anhydrase.It turned out that the bacterial strains C8 is a gram positive bacterium, which has a short pole shape and reaches the stable stage in 18 hours.And the identification result of 16S rRNA is the Bacillus sp.The carbonic anhydrase activity of the Bacillus sp have good thermal stability.Neutral and alkaline of the environment was fit for keeping stability of CA.A certain range of concentration of mental ions such as Ca2+, Mg2+, Zn2+ and Fe2+ along with anions such as SO2-4 and Cl-have few effects on the CA activity of B.RA51.This research provides scientific basis for applying the B.RA51's CA in circulating cooling water.关键词
喀斯特地貌/碳酸酐酶/循环冷却水Key words
Karst landform/carbonic anhydrase/circulating cooling water分类
资源环境引用本文复制引用
易哲,叶姜瑜,李大荣,窦建军,石玉竹..喀斯特地貌中碳酸酐酶微生物鉴定与特性研究[J].重庆理工大学学报（自然科学版）,2017,31(8):113-119,7.基金项目
国家科技重大专项(2013ZX07104004) （2013ZX07104004）
