动物营养学报2017，Vol.29Issue(2)：536-546,11.DOI:10.3969/j.issn.1006-267x.2017.02.021
花生四烯酸对日本沼虾肝胰腺细胞脂质代谢基因表达的影响
Effects of Arachidonic Acid on Lipid Metabolism-Related Gene Expressions of Hepatopancreas Cells Isolated from Juvenile Oriental River Prawn, Macrobrachium nipponense
摘要
Abstract
This experiment was conducted to determine the effects of arachidonic acid (ARA) concentration in culture medium on cell viability and lipid metabolism-related gene expressions of hepatopancreas cells isolated from juvenile oriental river prawn, Macrobrachium nipponense.The hepatopancreas cells were dissected from prawns and were cultured with complete culture medium for 5 days.After that, cultured cells were incubated in medium supplemented with graded levels [0 (ARA1) , 50 (ARA2), 100 (ARA3) , 200 (ARA4) and1 000 μmol/L (ARA5) of ARA.Cell viability at 24 h and gene expressions of lipid metabolism-related genes at 12 and 24 h were examined.The results showed as follows: the hepatopancreas cells showed well growth in complete culture medium, and could survive for 15 days;cell viability was significantly decreased by incubation with higher level (1 000 μmol/L) of ARA (ARA5 group) compared with ARA1 and ARA2 groups (P<0.05) after 24 h;higher level (1 000 μmol/L) of ARA (ARA5 group) caused significant decreases of gene expressions of delta-4 fatty acyl desaturase (Δ4 FAD), delta-6 fatty acyl desaturase (Δ6 FAD), very-longchain fatty acids-6 (Elovl6), scavenger receptor class B type Ⅰ (SR-B Ⅰ), fatty acid-binding protein 10(FABP10) and acyl-CoA binding protein (ACBP) of hepatopancreas cells incubation for both 12 and 24 h;after incubation with ARA for 12 h, the gene expression of SR-B Ⅰ of ARA2 group was significantly higher than that of other groups (P<0.05), FABP10 gene expression of ARA2 and ARA3 groups was significantly higher than that of ARA1 and ARA5 groups (P<0.05), and ACBP gene expression of ARA3 group was significantly higher than that of other groups (P<0.05);after incubation with ARA for 24 h, the highest expressions of SR-B Ⅰ , FABP10 and ACBP were observed in ARA2 group, which was significantly higher than those of other groups (P<0.05).These findings suggest that ARA can influence cell viability and lipid metabolism-related gene expressions of hepatopancreas cell isolated from Macrobrachium nipponense.Cell viability can be decreased by incubation with higher level of ARA (1 000 μmol/L).Appropriate levels of ARA (50 to100 μmol/L) can promote the expressions of genes related to fatty acyl desaturase, elongases of very-longchain fatty acids and fatty acid transport.[Chinese Journal of Animal Nutrition, 2017, 29 (2) : 536-546]关键词
日本沼虾/花生四烯酸/细胞培养/基因表达Key words
Macrobrachium nipponense/arachidonic acid/cell culture/gene expression分类
农业科技引用本文复制引用
丁志丽,曹访,罗娜,孔有琴,张易祥,李景芬,叶金云..花生四烯酸对日本沼虾肝胰腺细胞脂质代谢基因表达的影响[J].动物营养学报,2017,29(2):536-546,11.基金项目
国家自然科学基金(31402308) （31402308）
浙江省自然科学基金(LQ14C190004) （LQ14C190004）
浙江省重大科技专项计划项目(2014C02011) （2014C02011）
浙江省重点研发计划项目(2015C03018) （2015C03018）
