动物营养学报2017，Vol.29Issue(2)：547-555,9.DOI:10.3969/j.issn.1006-267x.2017.02.022
饲粮能氨水平对不同品种猪肉质性状及相关基因表达的影响
Effects of Dietary Energy and Nitrogen Levels on Meat Traits and Its Related Gene Expression in Different Strains of Pigs
摘要
Abstract
This study was conducted to investigate the effects of dietary energy and nitrogen levels on meat traits, muscle fiber type and cytokine related gene expression of pigs.Forty-eight pure-bred Bama mini-pigs and forty-eight Landrace pigs were selected and randomly allocated to four groups: Landrace pigs were fed NRC diet, Landrace pigs were fed Chinese feeding standard of swine (GB) diet, Bama mini-pigs were fed NRC diet and Bama mini-pigs were fed GB diet.The animals were fed from 5 weeks of age to their market weights.At the end of each stage (nursery, growing and finishing stages), eight pigs with similar body condition from each group were slaughted, and analysis the muscle samples.The results showed that the growth stages significantly affect the cooking yield percentage and mRNA expression levels of myosin heavy chain (MyHC) Ⅱx, MyHC Ⅱ b and interleukin-15 (IL-15) of longissimus dorsi muscle (LDM) (P<0.05), and it shows a increasing trend with growth stage.The cooking yield percentage, mRNA expression levels of MyHCⅠ of LDM of Bama mini-pigs were significantly higher than those of Landrace pigs (P<0.05), while the drip loss of LDM was significantly lower (P<0.05).Compared with the NRC diet, the GB diet significantly increased the pH45 min of LDM during growing stage (P<0.05), and significantly up-regulated mRNA expression level of MyHC Ⅰ of LDM at nursery and finishing stages (P<0.05);compared with the GB diet, the NRC diet significantly up-regulated mRNA expression level of MyHC Ⅰ of LDM at growing stage (P<0.05).Collectively, these findings suggest that strain and growth stage have significant effects on meat traits and its related gene expression levels of pigs.Dietary energy and nitrogen levels mainly through interacted with growth stage to affect meat quality and gene expression of muscle fiber type.[Chinese Journal of Animal Nutrition, 2017,关键词
肉质性状/肌纤维类型/细胞因子/巴马香猪/饲粮能氮水平Key words
meat traits/muscle fiber type/cytokine/Bama mini-pigs/dietary energy and nitrogen levels分类
农业科技引用本文复制引用
刘莹莹,李凤娜,李颖慧,印遇龙,孔祥峰..饲粮能氨水平对不同品种猪肉质性状及相关基因表达的影响[J].动物营养学报,2017,29(2):547-555,9.基金项目
国家973课题(2012CB124704,2013CB127305) （2012CB124704,2013CB127305）
湖南省自然科学基金项目(S2014J5041) （S2014J5041）
