动物营养学报2017，Vol.29Issue(2)：583-589,7.DOI:10.3969/j.issn.1006-267x.2017.02.026
饲料中添加活性酵母制剂对刺参生长、免疫力和抗病力的影响
Effects of Dietary Active Yeast Products on Growth, Immunity and Disease Resistance of Sea Cucumber (Apostichopus japonicas Selenka)
摘要
Abstract
This experiment was conducted to investigate the effects of dietary active yeast products on growth,immunity and disease resistance of sea cucumber (Apostichopus japonicas Selenka).Sea cucumbers with an average initial body weight of (4.0±-0.1) g were fed with 6 experimental diets according to a single factor experimental design: a commercial formula feed supplemented with 0 (control), 0.025％, 0.050％, 0.100％,0.200％ and 0.400％ active yeast products for 6 weeks.There were 3 replicates per diet with 15 sea cucumbers per replicate.After 6 weeks of feeding trial, a total of 10 sea cucumbers of each replicate were selected and injected into the body wall with the 1.5 × 109 CFU/mL Vibrio splendidus bacterial stock solution at a dose of0.1 mL per sea cucumber, and to observe and record the 14-day accumulative morbidity after challenged with Vibrio splendidus.The results showed as follows: supplementation of active yeast products could significantly enhance the specific growth rate (SGR) (P< 0.05), and the highest SGR of sea cucumbers were found in0.200％ active yeast products group.Sea cucumbers fed diet with active yeast products could significantly increase the activity of catalase (CAT) in coelomic fluid (P<0.05).Compared with the control group, active yeast products supplemented at 0.050％ to 0.400％ significantly enhanced the activity of lysozyme (LZM)(P<0.05) in coelomic fluid, supplemented at 0.200％ to 0.400％ significantly enhanced the activity of acid phosphatase (ACP) (P<0.05) in coelomic fluid, and supplemented at 0.400％ significantly enhanced the activity of superoxide dismutase (SOD) in coelomic fluid (P<0.05).Supplementation of active yeast products could decrease the cumulative mortality of sea cucumbers challenged with Vibrio splendidus after 14 days, and the lowest cumulative mortality of sea cucumbers was found in 0.200％ active yeast products group.It can be concluded that the supplementation of active yeast products in diets can enhance the growth, immunity and disease resistance of sea cucumbers in the condition of this experiment.Comprehensive analysis of growth, immunity and disease resistance of sea cucumbers and according to the production practice, the optimal supplemental level of active yeast products in the diet of sea cucumbers is 0.200％ to 0.400％.[Chinese Journal of Animal关键词
活性酵母制剂/刺参/生长/免疫力/抗病力Key words
active yeast products/sea cucumber/growth/immunity/disease resistance分类
农业科技引用本文复制引用
严芳,胡炜,李成林,张艳萍,赵斌,韩莎..饲料中添加活性酵母制剂对刺参生长、免疫力和抗病力的影响[J].动物营养学报,2017,29(2):583-589,7.基金项目
山东省现代农业产业体系刺参产业创新团队建设(SDAIT-22-01,SDAIT-22-05) （SDAIT-22-01,SDAIT-22-05）
山东省科技发展计划(2014GNC111022) （2014GNC111022）
山东省农业良种工程(2014-2016) （2014-2016）
国家海洋局海洋公益性行业科研专项经费(201305001-4) （201305001-4）
山东省农业科技成果转化资金项目(2014-2016) （2014-2016）
