动物营养学报2017，Vol.29Issue(2)：605-612,8.DOI:10.3969/j.issn.1006-267x.2017.02.029
不同品种猪在不同生理阶段骨骼肌葡萄糖转运体和胰岛素受体表达的发育性变化
Ontogenetic Regulation of Glucose Transporter and Insulin Receptor mRNA Expression in Skeletal Muscle of Different Kinds of Pigs at Different Physiological Stages
摘要
Abstract
This experiment was conducted to determine the ontogenetic regulation of glucose transporter(GLUT) 1, GLUT4 and insulin receptor (IR) mRNA expression in skeletal muscle of different kinds of pigs at different physiological stages.Lantang (LT) and Landrace (LD) pigs at the age of 1, 7, 30, 60, 90 and150 days were selected to determine the expression patterns of GLUT1 and GLUT4 at different skeletal muscle tissues including longissimus dorsi muscles (LM), semimembranosus muscles (SM) and semitendinosus muscles (SD) by the method of real-time semiquantitative PCR for cDNA templates.The results showed as follows : 1) LT and LD pigs exhibited similar development of mRNA expressions of GLUT1 in the LM, SM and SD from 1 to 150 days of age with the highest level at the age of 1 day.2) LT and LD pigs exhibited waveform variations of mRNA relative expression abundance of GLUT4 from 1 to 150 days of age with breed differences in the speed and extend of variations.At the age of 1 and 150 days, the mRNA relative expression abundance of GLUT4 in the LM of LT pig was lower than that of LD pig with the same age (P>0.05), while was higher than that of LD pig with the same age of the rest days (P>0.05).3) LT and LD pigs exhibited higher level of the mRNA relative expression abundance of IR in the LM, SM and SD at the age of 1 and 30 days,while there were not significant difference among 7, 60, 90 and 150 days of age (P>0.05).There was no significant difference in mRNA relative expression abundance of IR between LT and LD pigs with the same ages(P>0.05).The results suggest that GLUT1 mRNA expression in skeletal muscles of pigs can be regulated by physiological stages, while GLUT4 mRNA expression is not only differentially regulated by breeds but also differentially distributed in physiological stages, and the expression level of GLUT4 is weakly related to the IR mRNA expression and shows no apparent rules.[Chinese Journal of Animal Nutrition, 2017, 29 (2) : 605关键词
猪/生理阶段/葡萄糖转运载体1/葡萄糖转运载体4/胰岛素受体/发育规律Key words
pigs/physiological stages/glucose transporter 1/glucose transporter 4/insulin receptor/ontogenetic regulation分类
农业科技引用本文复制引用
刘文佐,冯定远,左建军,代发文,叶慧,曹庆云,周响艳,邹仕庚,黄志毅,职爱民..不同品种猪在不同生理阶段骨骼肌葡萄糖转运体和胰岛素受体表达的发育性变化[J].动物营养学报,2017,29(2):605-612,8.基金项目
国家重点基础研究发展计划项目(2012CB124701) （2012CB124701）
广东省科技计划项目(2016A020210104) （2016A020210104）
广州市科技计划项目(201510010258) （201510010258）
