动物营养学报2017，Vol.29Issue(2)：613-619,7.DOI:10.3969/j.issn.1006-267x.2017.02.030
不同氮能比饲粮对妊娠环江香猪脐带血管发育相关基因表达的影响
Effects of Different Nitrogen/Energy Diets on Expression of Genes Related to Umbilical Vessel Development of Pregnant Huanjiang Mini-Pigs
摘要
Abstract
This study was conducted to investigate the effects of different nitrogen/energy diets on expression of genes related to umbilical vessel development of pregnant Huanjiang mini-pigs.A total of 48 primiparous Huanjiang mini-pigs were randomly assigned into two groups according to the body weight (BW), and reared in eight replicates (pens) with three mini-pigs per replicate.After service, the animals were fed with high nitrogen/energy diet (digestible energy was 14.73 MJ/kg, crude protein content was 13.11％, nitrogen/energy was 0.89) and low nitrogen/energy diet (digestible energy was 12.24 MJ/kg, crude protein content was9.77％, nitrogen/energy was 0.80), respectively.At 45, 75 and 110 d of gestation, one pig was randomly selected and sacrificed from each pen.The umbilical cord tissues were collected from three fetuses with maximal BW, average BW and minimal BW per litter.The expression levels of fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor 2 (VEGFR2) and endothelial nitric oxide synthase (eNOS), which related to umbilical vessel development, were detected by RT-qPCR technique.The results showed that compared with the low nitrogen/energy diet group, the high nitrogen/energy diet significantly increased the expression levels of genes related to umbilical vessel development (P <0.05), including VEGF and VEGFR2 genes expression levels of fetus at 45 d of gestation with maximal BW;VEGF gene expression level of fetus at 45 d of gestation with average BW;VEGF, VEGFR2 and eNOS genes expression levels of fetus at 75 d of gestation with maximal BW;VEGF and eNOS genes expression levels of fetus at 75 d of gestation with average BW, VEGFR2 gene expression level of fetus at 75 d of gestation with minimal BW;FGF, VEGFR2 and eNOS genes expression levels of fetus at 110 d of gestation with maximal BW, FGF and VEGFR2 genes expression levels of fetus at 110 d of gestation with average BW, and VEGF and eNOS genes expression levels of fetus at 110 d of gestation with minimal BW.The expression levels of umbilical vessel genes of fetuses in different gestation stages with the same standard BW were different, as well as of fetuses with different standard BW in a same gestation stage.These findings suggest that the high nitrogen/energy diet containing higher levels of protein and amino acids can significantly increase the expression levels of genes related to the umbilical vessel development in pregnant Huanjiang mini-pigs, and the tested gene expression levels are different during different gestation stages, as well as among the fetuses with different standard body weights during a same gestation stage.[Chinese Journal of Animal Nutrition, 2017, 29(2) :613-619]关键词
环江香猪/妊娠/饲粮氮能比/脐带/血管发育Key words
Huanjiang mini-pigs/pregnancy/dietary nitrogen/energy ratio/umbilical cord/blood vessel development分类
农业科技引用本文复制引用
祝倩,马思聪,姬玉娇,印遇龙,孔祥峰..不同氮能比饲粮对妊娠环江香猪脐带血管发育相关基因表达的影响[J].动物营养学报,2017,29(2):613-619,7.基金项目
国家自然科学基金面上项目(31270044,31572421) （31270044,31572421）
中科院"西部之光"人才培养计划重点项目 （）
