动物营养学报2017，Vol.29Issue(2)：620-627,8.DOI:10.3969/j.issn.1006-267x.2017.02.031
茶皂素对奶牛瘤胃原虫区系的影响
Effects of Tea Saponin on Rumen Protozoa Flora of Dairy Cows
摘要
Abstract
The objective of this study was to research the effects of tea saponin on rumen protozoa flora of dairy cows.Twelve healthy Holstein dairy cows [body weight was (550 ± 30) kg;milk yield was35 kg/(head · d);2 to 4 parities] were randomly allocated to one of four groups with 3 cows per group.Tea saponin [0 (control), 20, 30 and 40 g/(head · d)] was perfused to cows before morning feeding.The experiment lasted for 49 d with 14 d of adaptation period and 35 d of formal test period.Rumen fluid was collected by mouth sampler at 1 h before morning feeding every 7 d during formal test period.Denaturing gradient gel electrophoresis (DGGE) combined with 18S rDNA sequence analysis technique was used for rumen protozoa flora analysis.The results showed as follows: 1) tea saponin could selectively inhibit protozoa growth.2)Compared with control group, richness index and Shannon index in 30 and 40 g/(head · d) tea saponin groups were significantly reduced (P<0.05), and dominance index was significantly increased (P<0.05), but evenness index was not significantly different among groups (P>0.05);tea saponin could significantly reduce rumen Vestibuliferia Entodiniomorphida protozoa number.In conclusion, dietary supplementation of 30 and40 g/(head · d) tea saponin can inhibit protozoa growth in rumen of dairy cows, and reduce the diversity of rumen protozoa.[Chinese Journal of Animal Nutrition, 2017, 29(2) :620-627]关键词
奶牛/原虫区系/茶皂素Key words
dairy cow/protozoa flora/tea saponin分类
农业科技引用本文复制引用
赵士萍,严淑红,蒋琦晖,常肖肖,王炳,方洛云,蒋林树,熊本海..茶皂素对奶牛瘤胃原虫区系的影响[J].动物营养学报,2017,29(2):620-627,8.基金项目
"十二五"国家科技支撑项目(25012BAD14B09) （25012BAD14B09）
"十三五"国家重大科技专项(2016YFDO70020,2016YFDO700205) （2016YFDO70020,2016YFDO700205）
北京市农业局北京市现代农业产业技术体系奶牛创新团队 （）
