动物营养学报2017，Vol.29Issue(2)：652-662,11.DOI:10.3969/j.issn.1006-267x.2017.02.035
胰岛素受体-1在尼罗罗非鱼不同组织中的表达及其对注射葡萄糖的响应
Expression of Insulin Receptor-1 in Different Tissues of Nile Tilapia and Its Response to Glucose Injection
摘要
Abstract
This experiment was conducted to determine the expression of insulin receptor (IR)-1 in different tissues of Nile tilapia (Oreochromis nilotica) and its response to glucose injection.A partial cDNA of IR-1 was cloned from muscle of Nile tilapia by real-time PCR method.The expression differences of IR-1 in muscle, liver and heart were compared by semi-quantitative PCR detection.A total of 160 Nile tilapia with the body weight about 100 g were randomly allotted to 2 groups with 4 replicates per group and 20 fish per replicate.In experimental group, glucose with the dose of 30 mg per 100 g body weight was injected intraperitoneally into the fish, while the fish in control group were injected with 0.7％ sterile saline water as the same dose and method.Samples were collected and measured before injection (0 h) and at 1, 3, 6 and 12 h after injection, respectively.The plasma glucose and insulin contents were analyzed, and the relative expression level of IR-1mRNA in muscle, heart and liver was measured by quantitative real-time PCR detection.The results show as follows: 1)the cloned partial cDNA of IR-1 (GenBank accession No.was JN967750) was 1 979 bp, and encoded 548 amino acids.Sequence analysis showed that IR-1 of Nile tilapia had high conservation compared with other species, and had a rich tyrosine kinase characteristic sequence.2) The higher expression level of IR-1 in muscle, heart and liver was found, the IR-1 expression level in liver and muscle was consistent, while that in heart was relatively lower.3) The maximum plasma glucose content of experimental group was observed at 1 h after glucose injection which was significantly higher than that of control group (P<0.05), and then began to decrease and returned to normal level at 3 h after injection;the maximum plasma insulin content of experimental group was observed at 3 h after glucose injection which was significantly higher than that of control group (P<0.05), and then began to decrease and returned to normal level at 12 h after injection.The relative expression level of IR-1 mRNA in muscle and liver at 6 h after glucose injection of experimental group was significantly higher than that of control group (P<0.05), and then to return to normal level at 12 h after injection;the relative expression level of IR-1 mRNA in heart had no significant change after glucose injection of experimental group (P>0.05).The results suggest that injecting glucose can instantaneously increase the plasma glucose content of Nile tilapia, but the increase of plasma insulin content is relatively delayed compare with plasma glucose content, and the relative expression level of IR-1 in muscle and liver show a delayed increase relative to plasma insulin content, then the burden of glucose metabolism in Nile tilapia is increased.关键词
尼罗罗非鱼/葡萄糖/胰岛素/胰岛素受体-1Key words
Nile tilapia (Oreochromis nilotica)/glucose/insulin/insulin receptor-1分类
农业科技引用本文复制引用
刘含亮,王纪亭,万文菊,孙敏敏,孟晓,徐蒙蒙..胰岛素受体-1在尼罗罗非鱼不同组织中的表达及其对注射葡萄糖的响应[J].动物营养学报,2017,29(2):652-662,11.基金项目
国家自然科学基金项目(31472288) （31472288）
山东省科技发展计划项目(2014GGH210010) （2014GGH210010）
山东省高等学校科技计划项目(J09LC05) （J09LC05）
