动物营养学报2017，Vol.29Issue(2)：678-689,12.DOI:10.3969/j.issn.1006-267x.2017.02.038
植物乳杆菌对玉米秸秆和水稻秸秆体外发酵特性的影响
Effects of Lactobacillus plantarum on in Vitro Rumen Fermentation Characteristics of Maize Straw and Rice Straw
摘要
Abstract
The objective of this trial was to explore and the effects of Lactobacillus plantarum on the characteristics of in vitro ruminal fermentation of maize straw and rice straw in dairy cows.The trial was conducted as one-factor block experimental design, and four supplemental levels [0 (control), 0.25 × 107, 0.50 × 107 and0.75× 107 CFU/mL] of Lactobacillus plantarum were designed to analyze the influence on in vitro fermentation gas production (1, 2, 4, 6, 12, 24, 36 and 48 h), gas production parameters, dry matter degradability(DMD), neutral detergent fiber degradability (NDFD), and concentrations of volatile fatty acid (VFA) and ammonia nitrogen (NH3-N), as well as pH in fermentation fluid.The results showed that the supplementation of Lactobacillus plantarum could significantly increase theoretical maximum gas production and gas production at 1 to 24 h of maize straw (P<0.05), and the optimum supplemental level was 0.75×107 CFU/mL;the supplementation of Lactobacillus plantarum could significantly increase gas production at 36 to 48 h (P<0.05),and the optimum supplemental level was 0.25× 107 CFU/mL.NH3-N concentration of two substrates of in vitro fermentation was enhanced linearly and significantly with the improvement of supplemental level of LactobaciIlus plantarum (P<0.05).However, there were no significant differences on NDFD, DMD, and VFA (acetic acid, propionic acid, isobutyric acid, butyric acid and valeric acid) concentrations and pH of fermentation fluid with the change of supplemental levels of Lactobacillus plantarum (P > 0.05).The results suggest that adding Lactobacillus plantarum can promote in vitro fermentation and nitrogen metabolism, and maintain pH balance of maize straw and rice straw, the optimal supplemental levels of which are 0.75 × 107 and 0.25 ×107 CFU/mL, respectively.[Chinese Journal of Animal Nutrition, 2017, 29 (2) : 678-689]关键词
植物乳杆菌/体外发酵/瘤胃/奶牛/玉米秸秆/水稻秸秆Key words
Lactobacillus plantarum/in vitro fermentation/rumen/dairy cows/maize straw/rice straw分类
农业科技引用本文复制引用
陈亮,任傲,李斌,周传社,谭支良..植物乳杆菌对玉米秸秆和水稻秸秆体外发酵特性的影响[J].动物营养学报,2017,29(2):678-689,12.基金项目
娟姗牛生产性能与乳品质提升营养调控关键技术研究(西藏自治区财政专项) （西藏自治区财政专项）
国家"十二五"科技支撑计划(2012BAD14B17) （2012BAD14B17）
国家自然科学基金(31001024) （31001024）
中国科学院亚热带农业生态研究所青年人才领域项目(ISACX-LYQY-QN-1105) （ISACX-LYQY-QN-1105）
