中国耳鼻咽喉头颈外科2017，Vol.24Issue(7)：371-374,4.DOI:10.16066/j.1672-7002.2017.07.013
白细胞介素13在嗜酸粒细胞性慢性鼻-鼻窦炎合并鼻息肉组织中的表达及其与黏蛋白高分泌的相关性研究
Study on expression of IL-13 and correlation between IL-13 and MUC5AC in eosinophilic chronic rhinosinusitis with nasal polyps
摘要
Abstract
OBJECTIVE To explore expression of IL-13 in eosinophilic chronic rhinosinusitis with nasal polyps(EOS-CRSwNP) and further investigate the correlation between IL-13 and MUC5AC in EOS-CRSwNP. METHODS MUC5AC was detected in tissues from normal nasal mucosa and EOS-CRSwNP by immunohistochemistry and ELISA. We also used ELISA to detect the expression of IL-13 in normal nasal mucosa and EOS-CRSwNP. Correlation between IL-13 and MUC5AC in EOS-CRSwNP was investigated by correlation analysis. Secretion of MUC5AC in IL-13 incubated primary air-liquid interface(ALI)-cultured nasal polyp epithelial cells was examined by ELISA. RESULTS MUC5AC is mainly expressed in the epithelium of nasal mucosa by immunohistochemistry. By ELISA, expressions of MUC5AC and IL-13 are higher in EOS-CRSwNP than those in normal nasal mucosa. Correlation analysis shows that there exists a high correlation between MUC5AC and IL-13 in EOS-CRSwNP. IL-13 upregulates expression of MUC5AC in IL-13 incubated primary ALI-cultured nasal polyp epithelial cells for 7 or 14 days. CONCLUSION Expressions of MUC5AC and IL-13 are higher in EOS-CRSwNP than those in normal nasal mucosa respectively, and MUC5AC correlates with IL-13 highly in EOS-CRSwNP.关键词
嗜酸细胞/鼻息肉/白细胞介素13/黏蛋白5ACKey words
Eosinophils/Nasal Polyps/Interleukin-13/mucin 5AC引用本文复制引用
张宇,王向东,王鸿,范尔钟,李颖,宋西成,张罗..白细胞介素13在嗜酸粒细胞性慢性鼻-鼻窦炎合并鼻息肉组织中的表达及其与黏蛋白高分泌的相关性研究[J].中国耳鼻咽喉头颈外科,2017,24(7):371-374,4.基金项目
长江学者和创新团队(IRT13082),国家自然科学基金重点国际合作研究项目(81420108009),科技部"十二五"国家科技支撑计划课题(2014BAI07B04),国家自然科学基金(81100704、81441029、81441031、81400444、81570894、81670907),首都医学发展基金(2011-1017-06),北京市卫生系统高层次人才计划(2009-2-007、2011-3-039、2011-3-043、2014-3-018),北京市医管局使命计划(SML20150203),北京市自然科学基金(7172054)和交叉学科合作项目(xxjc201712)联合资助 （IRT13082）
