中国耳鼻咽喉头颈外科2017，Vol.24Issue(8)：385-389,5.DOI:10.16066/j.1672-7002.2017.08.001
17-AAG联合EGCG对人鼻咽癌细胞株增殖和凋亡的影响及机制研究
Effect and mechanism of heat shock protein inhibitors on cell proliferation and apoptosis in human nasopharyngeal carcinoma
摘要
Abstract
OBJECTIVE To study the effect of different concentrations of 17-AAG and EGCG monotherapy or in combination on the induced apoptosis in human nasopharyngeal carcinoma, and to explore new molecular targets for the treatment of nasopharyngeal carcinoma. METHODS MTT colorimetric method and fluorescent staining were used to detect the change of CNE proliferation inhibition rate and cell morphology. And furthermore, the expression level of Bcl-2, Bax, Caspase-3 were detected by RT-PCR. RESULTS 1. 17-AAG or EGCG alone had inhibitory effect on the human nasopharyngeal carcinoma CNE cells at 24 h, 48h and 72 h, and it was related with time and dose(P<0.01). The inhibition effect of combination of 17-AAG and EGCG was significantly increased,which was time and dose dependent(P<0.01). 2. RT-PCR was used to detect the mRNA expression level of Bcl-2, Bax and caspase-3. The level of Caspase-3 and Bax mRNA expression after treated by 17-AAG and EGCG was significantly higher, and the level of bcl-2 mRNA expression was lower than that after treated by 17-AAG or EGCG alone. CONCLUSION Our investigation implied that 17-AAG and EGCG in combination can effectively inhibit the proliferation of human nasopharyngeal carcinoma CNE cells. The involved mechanisms may be associated with the upregulation of Bax and Caspase-3 expression.关键词
鼻咽肿瘤/细胞增殖/细胞凋亡/逆转录聚合酶链反应Key words
Nasopharyngeal Neoplasms/Cell Proliferation/Apoptosis/Reverse Transcriptase Polymerase Chain Reaction引用本文复制引用
庞秋霞,何静子,赵菊梅,许静洪,王爱红,陈美霓,刘涛..17-AAG联合EGCG对人鼻咽癌细胞株增殖和凋亡的影响及机制研究[J].中国耳鼻咽喉头颈外科,2017,24(8):385-389,5.基金项目
陕西省教育厅专项科研计划项目(2013JK0783)和延安市科技创新团队建设(2015CXTD-10)联合资助 （2013JK0783）
