口腔疾病防治2017，Vol.25Issue(9)：554-559,6.DOI:10.12016/j.issn.2096-1456.2017.09.002
不同来源骨髓间充质干细胞成骨能力的比较
Comparison of osteogenic differentiation abilities of mesenchymal stem cells from different sources of hBMSCs
摘要
Abstract
Objective To compare the osteogenic differentiation abilities of human bone marrow mesenchymal stem cells (hBMSCs) from different sources, and to provide basis for choosing a new source of seed cells in bone tissue engi-neering. Methods Jaw bone-marrow-derived mesenchymal stem cells (JMMSCs) were isolated from orthognathic surgi-cal sites and cultured by limited dilution for single cell clone. Long bone-marrow-derived mesenchymal stem cells (BMMSCs) were obtained from bone marrow of volunteers and isolated by density gradient centrifugation method. Flow cytometry was used to detect the surface markers of both cells. Osteogenic ability was assessed by PCR and Western Blot after osteogenic differentiation for the following molecules:Runx2, COL-1 and OCN. Alizarin red staining was used for determining the ability of cell mineralization after osteogenic differentiation. Results The expressions of cell sur-face markers CD90 and CD105 were positive in both type of cells, while CD34, CD14 and CD45 were all negative. After 21 days of osteogenic induction, JMMSCs formed significantly more mineralized nodules than BMMSCs. After 7, 14, 21 days of osteogenic induction, JMMSCs expressed more osteogenic-related molecules than BMMSCs. Conclusion The osteogenic differentiation capacity and mineralization ability of JMMSCs are significantly higher than BMMSCs. Jaw bone might be a more suitable source of seed cells in bone tissue engineering compared with long bone.关键词
骨髓间充质干细胞/颌骨/长骨/成骨分化/种子细胞Key words
Bone marrow mesenchymal stem cells/Jaw bone/Long bone/Osteogenic differentiation/seed cell分类
生物科学引用本文复制引用
袁林,钱钧,杨征毅,王涵,郭武城,程洁莉,宋晶晶,何恩亮,张忆..不同来源骨髓间充质干细胞成骨能力的比较[J].口腔疾病防治,2017,25(9):554-559,6.基金项目
广州市科信局科技惠民专项资助(2014Y2-00058) （2014Y2-00058）
广州市属高校科研计划科技类一般项目(2012C096) （2012C096）
